异丙酚和异氟醚对大鼠全脑缺血再灌注损伤的影响

目的：观察异丙酚和异氟醚对大鼠全脑缺血再灌注损伤的影响。方法：雄性SD大鼠随机分为2组，每组14只，分别在异丙酚和异氟醚麻醉下行全脑缺血15min，每组各7只于再灌注后30min和3d测SOD活性和MDA含量或海马CA1区存活神经元计数。结果：异丙酚组大鼠海马和皮层SOD活性显著高于异氟醚组（P〈0，01），MDA含量显著低于异氟醚组（P〈0．01）。异丙酚组海马CA1区成活神经元计数显著多于异氟醚组（P〈0．05）。结论：异丙酚对大鼠脑缺血再灌注损伤的保护作用强于异氟醚，其机制与其减轻氧自由基损伤有关。

Effects of propofol and isoflurane on general brain ischemia-reperfusion injury in rats

AIM: To explore the effects of propofol and isoflurane on total cerebral ischemia-reperfusion injury of rats. METHODS: Male SD rats were randomly divided into two grougs (n=14), which were respectively anesthetized with propofol and isoflurane under mechanical ventilation. Global cerebral ischemia was induced by occlusions of bilateral common carotid and vertebral arteries (four vessels). Each group was divided into two subgroups according to reperfusion time: 30 min for detecting MDA and SOD in brain tissues and 3 d for detecting survived CA1 Cells of hippocampus. RESULTS: The contents of MDA was more after 30 min reperfusion in isoflurane group than that in propofol group(P< 0.01 ), whereas the activities of SOD was higher in propofol group than that in isoflurane group(P< 0.01 ). There were more survived neurons in CA1 after 3 day reperfusion in propofol group than in isoflurane group(P< 0.05 ). CONCLUSION: Propofol has stronger protection effect in general brain ischemia-reperfusion injury, the mechanism is associated with the activity of oxide free radical.