DNA指纹图谱与测序技术在中药品质鉴定中的研究概述 |
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引用本文: | 仇萍,盛孝邦,罗杰英. DNA指纹图谱与测序技术在中药品质鉴定中的研究概述[J]. 中医药导报, 2006, 12(11): 77-79 |
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作者姓名: | 仇萍 盛孝邦 罗杰英 |
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作者单位: | 湖南正清制药集团股份有限公司,湖南,长沙,410015;湖南农业大学农学院,湖南,长沙,410128;湖南中医药大学,湖南,长沙,410007 |
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摘 要: | DNA指纹图谱可从分子水平对中药材的种质进行准确的鉴定。文章介绍了随机扩增多态性DNA技术、限制性片段长度多态性分析技术、mRNA差异显示技术、微卫星DNA技术、单核苷酸多态性技术在中药品质鉴定的应用。
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关 键 词: | DNA指纹图谱 测序技术 中药鉴定 综述 |
文章编号: | 1672-951X(2006)11-0077-03 |
收稿时间: | 2006-04-25 |
修稿时间: | 2006-04-25 |
Overview on the Studies of DNA Finger Printing and Sequencing Techniques in Qualitative Determination of Chinese Crude Drugs |
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Abstract: | The DNA finger printing can identify the quality of Chinese crude drugs accurately from molecular level. The paper introduce some correlative techniques which were applied in identification of Chinese crude drugs, such as randomly amplified polymorphic DNA, restriction fragment length polymorphism, mRNA -- differential display technique, Sequence - tagged microsatellites, single nucleotide polymorphism. |
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Keywords: | DNA finger printing Sequencing Techniques Identification of Chinese crude drugs Summarization |
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