脑脊液外泌体lncRNA SOCS2-AS1预测脑胶质瘤复发的价值

目的 探讨脑脊液lncRNA SOCS2-AS1预测脑胶质瘤术后复发的价值。方法 选取2017年1月至2018年1月手术治疗的脑胶质瘤84例（高级别胶质瘤46例，低级别胶质瘤38例），另选取将康体检者30例为对照，腰椎穿刺术取脑脊液并分离外泌体，用RT-PCR检测外泌体lncRNA SOCS2-AS1水平。以lncRNA SOCS2-AS1相对表达量中位数为截断值，将胶质瘤分为高表达组和低表达组。结果 胶质瘤脑脊液外泌体lncRNA SOCS2-AS1相对表达量明显高于对照组（P<0.05），而且高级别胶质瘤脑脊液lncRNA SOCS2-AS1相对表达量明显高于低级别胶质瘤（P<0.05）。多因素logistic回归分析显示，脑脊液外泌体lncRNA SOCS2-AS1高表达是脑胶质瘤术后复发的独立影响因素（P<0.05）。ROC曲线分析显示，脑脊液外泌体lncRNA SOCS2-AS1高表达预测脑胶质瘤术后复发的曲线下面积为0.917，灵敏度为85.78%，特异度为93.10%。结论 脑胶质瘤病人脑脊液外泌体lncRNA SOCS2-AS1表达水平升高；脑脊液外泌体lncRNA SOCS2-AS1高表达对脑胶质瘤术后复发有较高的的预测价值。

Predictive value of cerebrospinal fluid exosomal lncRNA SOCS2-AS1 in postoperative recurrence of human brain glioma

Objective To investigate the predictive value of cerebrospinal fluid (CSF) exosomal lncRNA SOCS2-AS1 in predicting postoperative recurrence of human brain glioma. Methods Eighty-four patients with glioma (46 high-grade glioma and 38 low-grade glioma) who underwent surgery from January 2017 to January 2018 were selected, and 30 health examination subjects were selected as control. CSF was extracted by lumbar puncture and exosomes were isolated. The level of exosomal lncRNA SOCS2-AS1 was detected by RT-PCR. The glioma patients were divided into high expression group and low expression group according to the median expression level of lncRNA SOCS2-AS1. Results The expression level of CSF exosomal lncRNA SOCS2-AS1 of glioma was significantly higher than that of control group (P<0.05), and the expression level of CSF exosomal lncRNA SOCS2-AS1 of high-grade glioma was significantly higher than that of low-grade glioma (P<0.05). Multivariate logistic regression analysis showed that high expression of CSF exosomal lncRNA SOCS2-AS1 was an independent risk factor for postoperative recurrence of glioma (P<0.05). ROC curve analysis showed that the area under the curve of high expression of CSF exosomal lncRNA SOCS2-AS1 for predicting postoperative recurrence of glioma was 0.917, with a sensitivity of 85.78% and a specificity of 93.10%. Conclusions The expression level of CSF exosomal lncRNA SOCS2-AS1 is up-regulated in patients with glioma. The high expression of CSF exosomal lncRNA SOCS2-AS1 has a high predictive value for postoperative recurrence of glioma.