Spinophilin的表达与垂体腺瘤侵袭性的关系

目的 探讨垂体腺瘤（PA）组织Spinophilin（SPN）的表达及其与腺瘤侵袭性的关系。方法 选取2018年8月至2021年6月手术切除并经术后病理确诊的PA组织98例和瘤旁组织64例，用免疫组化染色检测SPN蛋白表达水平，应用PCR检查PA组织SPN mRNA表达水平。根据Knosp分类法评估肿瘤侵袭性。结果 98例中，侵袭性46例，非侵袭性52例。PA组织SPN阳性表达率（21.43%，21/98）明显低于瘤旁组织（71.87%，46/64；P<0.05）。侵袭性PA组织SPN阳性表达率（13.04%，6/46）明显低于非侵袭性PA组织（28.85%，15/52；P<0.05）。PA组织SPN mRNA相对表达量（1.21±0.37）明显低于瘤旁组织（2.89±0.75；P<0.05）。SPN阴性表达（OR=2.876；95%CI 1.003~5.121；P<0.001）是PA侵袭性的独立危险因素。ROC曲线分析显示，SPN mRNA预测PA侵袭性的AUC为0.830（95%CI 0.813~0.916；P<0.001），诊断临界值为0.84，灵敏度和特异度分别为95.23%和83.12%。结论 侵袭性PA组织SPN呈低表达，检测SPN表达水平对PA侵袭性有一定评估作用。

Expression of Spinophilin in pituitary adenoma tissues and its relationship with tumor invasiveness

Objective To investigate the relationship of Spinophilin (SPN) expression in pituitary adenoma (PA) tissues and tumor invasiveness. Methods The SPN expression was detected in PA tissues obtained from 98 PA patients who underwent microsurgery from August 2018 to June 2021 and in 64 paratumor tissues. The expression level of SPN mRNA was detected by real-time fluorescent PCR in PA tissues. PA invasiveness was assessed by Knsop grdea. Results Of 98 PA patients, 46 patients were classified as invasive PA and 52 non-invasive. The positive rate of SPN expression in paratumor tissues (71.87%, 46/64) was significantly higher than that in PA tissues (21.43%, 21/98; P<0.05). The positive rate of SPN expression in invasive PA tissues (13.04%, 6/46) was signifcnatly lower than that (28.85%, 15/52) in non-invasive PA tissues (P<0.05). The relative expression of SPN mRNA in PA tissues (1.21±0.37) was significantly lower than that (2.89±0.75) in paratumor tissue (P<0.05). The relative expression of SPN mRNA in invasive PA tissues (0.73±0.16) was significantly lower than that (1.68±0.80) in non-invasive PA tissues (P<0.05). Multivariate logistic regression analysis showed that negative expression of SPN was an independent risk factor of PA invasion (OR=2.876; 95%CI 1.003~5.121; P<0.05). ROC curve analysis showed that the AUC of SPN mRNA≥0.84 for predicting PA invasion was 0.830, with a sensitivity and specificity of 95.23% and 83.12%, respectively. Conclusions The expression of SPN in invasive PA tissues is low, and the detection of SPN expression has a certain value in assessing the invasiveness of PA.