垂体腺瘤组织miR-26b-5p表达与术后复发的关系

目的 探讨微小核糖核酸-26b-5p（miR-26b-5p）与垂体腺瘤术后复发的关系。方法 收集2018年1月~2020年1月手术切除的124例垂体腺瘤组织,采用qRT-PCR检测miR-26b-5p相对表达水平。术后随访2年,利用多因素logistic回归模型分析垂体腺瘤术后复发的危险因素,绘制ROC曲线分析miR-26b-5p评估垂体腺瘤术后复发的效果。结果 124例中,术后复发34例,复发率为27.42%（34/124）。复发垂体腺瘤miR-26b-5p相对表达量（1.25±0.43）明显低于非复发垂体腺瘤[（1.87±0.49）;P<0.001]。多因素logistic回归分析显示,miR-26b-5p低表达是术后复发的独立危险因素（OR=1.232;95% CI 1.058~3.524;P<0.001）。ROC曲线分析显示,miR-26b-5p表达水平评估垂体腺瘤术后复发的曲线下面积为0.841（95% CI 0.767~0.915;P<0.001）,最佳截断值为1.518,敏感度为84.80%,特异度为81.00%。结论 复发垂体腺瘤的miR-26b-5p表达水平明显下降,miR-26b-5p表达变化与术后复发密切相关,对评估垂体腺瘤术后复发有一定的价值。

Expression of miR-26b-5p in pituitary adenoma tissues and its relationship with postoperative recurrence

Objective To investigate the expression of miR-26b-5p in pituitary adenoma tissues and its relationship with postoperative recurrence. Methods The expression of miR-26b-5p was detected using qRT-PCR in pituitary adenoma tissues obtained from 124 patients who underwent surgery from January 2018 to January 2020. All the patients were followed up for 2 years. Multivariate logistic regression model was used to analyze the risk factors of postoperative recurrence of pituitary adenoma. Receiver operating characteristic (ROC) curve was used to analyze the value of miR-26b-5p to assess postoperative recurrence. Results Of the 124 patients, 34 patients recurred after operation, and the recurrence rate was 27.42% (34/124). The relative expression of miR-26b-5p in recurrent pituitary adenomas (1.25±0.43) was significantly lower than that (1.87±0.49) in non-recurrent pituitary adenomas (P<0.001). Multivariate logistic regression analysis showed that low expression of miR-26b-5p was an independent risk factor for postoperative recurrence (OR=1.232; 95% CI 1.058~3.524; P<0.001). ROC curve analysis showed that the area under the curve of miR-26b-5p expression level in evaluating the postoperative recurrence of pituitary adenoma was 0.841 (95% CI 0.767~0.915; P<0.001), with a sensitivity of 84.80% and a specificity of 81.00%. Conclusions The expression level of miR-26b-5p in recurrent pituitary adenoma is significantly decreased, and the change of miR-26b-5p expression is closely related to postoperative recurrence, which has a certain value in evaluating postoperative recurrence of pituitary adenoma.