下调BAG3表达对胶质瘤U87细胞增殖和凋亡的影响

目的 探讨下调BAG3表达对胶质瘤U87细胞增殖和凋亡的影响。方法 体外培养U87细胞，转染pEGFP-BAG3-shRNA质粒构建BAG3低表达胶质瘤U87细胞株（低表达组），转染pEGFP-shRNA对照质粒为对照组；利用RT-PCR和免疫印迹法鉴定；利用CCK8和EdU检测U87细胞增殖，利用流式细胞术检测U87细胞凋亡。结果 与对照组相比，RT-PCR和免疫印迹法检测结果显示低表达组BAG3 mRNA和蛋白表达水平均明显降低（P<0.05），低表达组24、48、72 h细胞增殖水平均明显降低（P<0.05），低表达组细胞凋亡率明显增高（P<0.05）。结论 下调胶质瘤U87细胞BAG3表达显著抑制其增殖、促进其凋亡。

Effect of down-regulation of BAG3 on proliferation and apoptosis of glioma U87 cells

Objective To explore the effect of down-regulation of BAG3 on the proliferation and apoptosis of glioma U87 cells. Methods U87 cells with down-regulation of BAG3 were reconstruted by transfection of shBAG3 plasmids, and were identified by the RT-PCR and western blotting. The proliferation of U87 cells was tested by CCK8 and EdU assay, and the apoptosis of U87 cells was tested by flow cytometry. Results The results of RT-PCR and western blotting showed that mRNA and protein expression levels of BAG3 were significantly down-regulated after transfection of shBAG3 plasmids. After transfection of shBAG3 plasmids, CCK8 and EdU assay showed that the proliferation of U87 cells was significantly inhibited and flow cytometry showed that the apoptosis of U87 cells was significantly promoted. Conclusions Down-regulation of BAG3 inhibits proliferation and promotes apoptosis of glioma U87 cells.