肺表面活性蛋白-D在高原肺水肿发生发展中的表达及作用

目的探讨肺表面活性蛋白-D（SP-D）在高原肺水肿发生发展中的表达及作用。 方法将64只Wistar雄性大鼠随机分为4组，正常对照组（16只，586 m室温饲养），低压低氧24 h、48 h及72 h组（各16只，于模拟海拔6000 m低压低氧舱内分别饲养24 h、48 h及72 h），取右侧肺组织行HE染色，观察肺组织损伤情况，收集左侧肺组织肺泡灌洗液，采用酶联免疫吸附法（ELISA）测定支气管肺泡灌洗液（BALF）中SP-D、白介素（IL）-1β、IL-6、C反应蛋白（CRP）及肿瘤坏死因子α（TNF-α）的含量。 结果成功建造高原肺水肿动物模型；与正常对照组相比，低压低氧24、48、72 h组肺组织病理评分升高，差异有统计学意义（P＜0.05）；SP-D含量均有所下降，IL-1β、IL-6、CRP、TNF-α含量均上升，且各组间两两比较差异均有统计学意义（P＜0.05）；BALF中SP-D含量与肺组织病理评分呈负相关（r=-0.687，P＜0.05），炎症因子IL-1β、IL-6、CRP及TNF-α与肺组织病理评分均呈正相关（r=0.705，P＜0.05；r=0.728，P＜0.05；r=0.733，P＜0.05；r=0.737，P＜0.05）。 结论SP-D在高原肺水肿的发生发展中起着一定的作用，其可能是防治高原肺水肿的新靶点。

Expression and effect of pulmonary surfactant protein-D in the occurrence and development of high altitude pulmonary edema

ObjectiveTo investigate the expression and effect of pulmonary surfactant protein-D (SP-D) in the occurrence and development of high altitude pulmonary edema. Methods64 male Wistar rats were randomly divided into four groups: normal control group (n=16, 586 m altitude with room temperature feeding), low-pressure hypoxia 24 h, 48 h and 72 h group (n=16, feeding in a simulated altitude 6000 m with low-pressure hypoxia chamber for 24 h, 48 h and 72 h, respectively). The right lung tissue was used for HE staining to observe the lung tissue injury. The alveolar lavage fluid of the left lung was taken to measure the SP-D, IL-1β, IL-6, CRP and TNF-α by enzyme-linked immunosorbent assay. ResultsThe animal model of high-altitude pulmonary edema was successfully established. Compared with the control group, the pathological scores of lung tissue in the 24, 48 and 72 h hypoxia groups were increased. The protein expression of SP-D was decreased while IL-1β, IL-6, CRP and TNF-α were increased significantly (P＜0.05). SP-D in alveolar lavage fluid was negatively correlated with lung histopathological score (r=-0.687, P＜0.05). Inflammatory factors IL-1β, IL-6, CRP and TNF-were positively correlated with lung histopathological score (r=0.705, P＜0.05; r=0.728, P＜0.05; r=0.733, P＜0.05; r=0.737, P＜0.05). ConclusionSP-D plays a important role in the occurrence and development of high altitude pulmonary edema, which may be a new target for the prevention and treatment.