过氧化氢对生育男性精子凋亡及核DNA完整性的影响

目的研究体外过氧化氢对生育男性精子凋亡及精子DNA完整性的影响。方法58例正常生育男性均来自吉林大学第二临床医院泌尿男科，精子Percoll优选后制作精子悬液，用伊红Y染色分析精子活率，用瑞-姬染色分析精子凋亡率，用精子核吖啶橙荧光染色检测精子DNA完整性。结果外加过氧化氢浓度为0.02mmol/l时，精子活率显著低于对照组（P〈0.05）。当浓度为0.20mmol/l时，精子活率显著低于对照组（P〈0.05），精子凋亡率显著高于对照组（P〈0.05）。外加过氧化氢浓度为0.02mmol/l、0.2mmol/l时，精子DNA完整性均显著低于对照组（P〈0.05），且精子DNA完整性随实验浓度增加而下降。结论体外过氧化氢对精子活率、凋亡率与DNA完整性有影响，高浓度的过氧化氢可促使精子凋亡，损伤精子DNA完整性，导致男性不育。

Influence of H2O2 in vitro on human sperm viability and DNA integrity of fertile male

Objective： To study effect of H2O2 in vitro on human sperm viability and DNA integrity of fertile male. Methods： Semen samples were obtained from 58 fertile male in department of urology, the second hospital, Jilin University. Sperm viability was detected by eosin - Y staining. The semen smears were stained by Wright - Giemsa staining for analyzing spermapoptosis rate. Sperm DNA integrity was detected by sperm nucleus acridine orange fluorescence staining. Results： When H2O2 concentration in vitro was 0. 02mmol/l, sperm viability was markedly lower than that of control group （ P 〈 0. 05 ）. When it was 0. 02mmol/l, sperm viability was markedly lower than that of control group （ P 〈 0. 05 ）, sperm apoptosis rate were markedly higher than that of control group （ P 〈 0. 05 ）. When it were 0. 02mmol/l, 0. 2mmol/l, sperm DNA integrity was both markedly lower than that of control group （ P 〈 0. 05 ）. With H2O2 concentration increasing, sperm DNA integrity was descending. Conclusion： H2O2 in vitro influence on sperm viability, sperm apoptosis rate and DNA integrity. H2O2 of high concentration could make sperm apoptosis, and damage sperm DNA integrity.