Antibody-mediated cellular cytotoxicity against Raji cells ADCC(RAJI): Evaluation of false positives in the detection of circulating immune complexes by Raji-cell assay |
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| Authors: | Mrinal K Dasgupta Thavisakdi Kovithavongs Joy Schlaut Bryan M Longenecker John B Dossetor |
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| Institution: | (1) Department of Medicine, Division of Nephrology and Immunology, University of Alberta, T6G 2B7 Edmonton, Alberta, Canada;(2) University Hospital, Room 1046, T6G 2B7 Edmonton, Alberta, Canada;(3) Department of Immunology, University of Alberta, T6G 2B7 Edmonton, Alberta, Canada |
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| Abstract: | Raji-cell radioimmunoassay is a very sensitive and reproducible method for the detection of circulating immune complexes. Using a complement-independent, antibody-dependent cellular cytotoxicity assay against51Cr-labeled Raji cells, there is no correlation between activity against Raji cells and positivity in Raji-cell radioimmunoassay for circulating immune complexes in three sets of sera (from renal transplantation patients, multiparous women, and patients suffering from systemic lupus erythematosus). We conclude that IgG antibodies to Raji membranes are not a significant cause of false-positive results in circulating immune complexes as detected by Raji-cell radioimmunoassay. |
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| Keywords: | Circulating immune complexes (CIC) Raji-cell radioimmunoassay (Raji-RIA) false positivity by antilymphocytic antibodies ADCC(Raji) CDC(Raji) |
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