番泻叶不同粉体的化学成分和泻下作用比较

目的 研究番泻叶不同粉体的化学成分与泻下作用。方法 以燥结缺水便秘小鼠为模型，运用色素流动法，以黑便为指示，通过观测小鼠首次排黑便的时间、稀便点数等指标来比较说明番泻叶不同粉体的泻下作用强弱。利用HPLC法测定番泻叶不同粉体致泻主要活性成分番泻苷A、番泻苷B，比较各粉体中番泻苷A、B的量高低，反映药理活性强弱。结果 通过小鼠动物实验观测到，番泻叶超微饮片组首次排黑便的时间明显提前，比饮片组提前了76 min，比超微粉组提前了18 min；稀便点数增多，比药材饮片组多3.7个，比超微粉组多2.84个。HPLC法测定番泻苷A、B的量显示，超微饮片中番泻苷A和B的质量分数为0.43%，是药材饮片中的1.6倍。结论 番泻叶3种粉体的水煎液都能明显增加小鼠腹泻的发生率，尤其以番泻叶超微饮片的通便泻下作用最显著，番泻苷A、B的量最高。

Comparison between chemical constituents and efficacy of different powder in Sennae Folium

Objective To study the chemical constituents of different powder in Folium Sennae and their cathartic effect. Methods The model of constipation on mice was established by water-deprivation but food-intaking, while pigment flow method was applied and black defecation was taken as the index. By observing the first time of black defecation and the loose stools points of mice, etc, we can explain the strength of making diarrhea by different powder in Folium Sennae. By HPLC to determine sennoside A and sennoside B which were the main ingredients to make diarrhea for comparing the content and reflect the intensity of pharmacological activity of different powder in Sennae Folium. Results For the constipation mice, Sennae Folium ultramicro pieces could shorten the first defecation time obviously, 76 min earlier than Chinese herbal pieces, 18 min earlier than superfine powder. It could also increase the points of loose stools, 3.7 more than Chinese herbal pieces, 2.84 more than superfine powder. Determination of sennoside A and sennoside B by HPLC showed that the content of Sennae Folium ultramicro pieces is 0.43%, 1.6 times as high as Chinese herbal pieces. Conclusion Three kinds of decoction of Sennae Folium powder could significantly increase the incidence of diarrhea in mice, and especially the cathartic effect of ultramicro-fine pieces, in which the content of sennoside A and B is the highest, is much more obvious than that of Chinese herbal pieces and ultro-fine powder of Sennae Folium.