肝细胞癌中NOS和VEGF的表达及其与肿瘤血管形成的关系

目的 研究Ⅱ、Ⅲ型一氧化氮合酶（ｉＮＯＳ、ｃＮＯＳ）和血管内皮生长因子（ＶＥＧＦ）在肝细胞癌中的表达及其与肿瘤血管形成的关系。方法 应用免疫组化方法检测７１例肝细胞癌患者手术切除石蜡包埋标本ｉＮＯＳ、ｅＮＯＳ、ＶＥＧＦ的表达，抗ＣＤ３４单克隆抗体显示血管内皮细胞。根据ＣＤ３４阳性的血管内皮细胞计数来测定肿瘤微血管密度（ＭＶＤ）。结果 ｉＮＩＳ、ｅＮＯＳ分别在８１．３％、８５．９％的肝癌中阳性表达，

Nitric oxide synthase and vascular endothelial growth factor expression in hepatocellular carcinoma and their relation to angiogenesis

OBJECTIVE: To analyse inducible nitric oxide synthase (iNOS), endothelial nitric oxide synthase (eNOS), vascular endothelial growth factor (VEGF) protein expression in hepatocellular carcinoma (HCC) and their relation to angiogenesis. METHODS: Tissue sections from 71 HCC patients were examined immunohistochemically for protein expression of iNOS, eNOS, and VEGF. Microvessal density (MVD) was counted by endothelial cells immunostained by anti-CD34 antibody. RESULTS: Positive iNOS and eNOS immunostaining was detected in 83.1% and 85.9% of HCC respectively. iNOS and eNOS were not detected in normal hepatic tissue. MVD was 34.3 +/- 1.5/HP and 38.6 +/- 1.6/HP in HCC positive for iNOS and VEGF while it was 31.2 +/- 2.8/HP, and 22.4 +/- 2.0/HP in HCC negative for iNOS and VEGF respectively (P < 0.01). A correlation between NOS expression and VEGF in HCC was not observed. CONCLUSION: iNOS and eNOS may play a role in malignant transformation of post-hepatic cirrhosis. The expression of iNOS and VEGF favors angiogenesis of HCC.