Humanin对缺氧诱导的大鼠皮层神经元损伤的保护作用

目的观察Humanin（HN）对缺氧诱导皮层神经细胞损伤的保护作用。方法培养10d的原代皮层神经元在缺氧环境下（氧含量〈2％）放置4h诱导神经细胞损伤。实验组在缺氧之前24h分别加入终浓度为10μmol／L和20μmol／L的HN。通过测定MTT和Calcein—AM染色检测细胞活力。通过测定丙二醛（MDA）、超氧化物歧化酶（SOD）、乳酸脱氢酶（LDH）的变化检测细胞损伤的程度。结果HN可以提高缺氧4h后细胞的存活率，同时可以降低由细胞损伤所引起的MDA、SOD、LDH的升高。20μmol／L的HN具有比10μmol／L更显著的保护作用。结论HN对缺氧诱导的神经元细胞损伤具有保护作用。

The Neuroprotective Effects of Humanin on Hypoxia - induced Cortical Neurons Insults in Rats

Objective To study the neuroprotective effects of humanin on the hypoxia -induced cortical neurons insults. Methods Primary cortical neurons were cultured for 10 days and then were put in the hypoxic environment（oxygen concentration 〈2%） for 4 h. Humanin was added to culture medium at 10 μmol/L and 20 μmol/L final concentration at 24 h before treatment. The cell survival rate was calculated by MTT assay and Calcein- AM staining. The production of malonaldehyde （MDA）, the viability of superoxide dismutase（SOD） and lactate dehydrogenase（LDH） were assayed. Results Humanin could improve the cell viability after hypoxia for 4 h and prevented hypoxia- induced the increase of levels of MDA,SOD and LDH. 20 μmol/L humanin showed a stronger neuroprotective effect than 10 μmol/L humanin did. Conclusion Humanin had a neuroprotective effect on hypoxia - induced cortical neurons insults.