PAI-1 mRNA在糖尿病大鼠肾脏的表达与通心络胶囊的干预作用研究

目的研究纤溶酶原激活物抑制物-1（PAI-1）在糖尿病大鼠肾皮质的表达和中药通心络胶囊对其的干预作用。方法将SD大鼠随机分为两组,一组经链尿佐菌素诱发的糖尿病模型（DM）组,另一组为血糖正常对照组（C）,两组均再随机分为DMl组、DM2组、Cl组、C2组,其中DM2组、C2组给予通心络治疗5周。治疗前后称体重、监测血糖,最后取肾脏称重,采用逆转录一多聚酶链式反应（RT-PCR）观测肾皮质PAI-1mRNA表达量。结果 DM组大鼠肾重/体重值及PAI-lmRNA表达明显高于C组（P〈0.01）,DM2组PAI-1mRNA表达低于DMl组,差异有统计学意义（P〈0.05）,肾皮质PAI-1mRNA表达量在C1与C2组间无统计学意义（P〉0.05）。结论 PAI-1mRNA在糖尿病大鼠肾皮质的表达增高,PAI-1增高参与糖尿病肾病的病理生理过程。中药通心络对糖尿病时PAI-1mRNA肾皮质的表达增高有下调作用。

Expression of Plasminogen Activator Inhibitor-1 in Kidney of Diabetic Rats and Its Modulation by Tongxinluo Capsule

Objective To investigate the expression of plasminogen activator inhibitor（PAI-1） in renal codex of diabetic rats and the effect of Tongxinluo capsule on it.Methods Sprague-Dawley rats were randomly divided into two groups：Streptozotocin induced diabetic model group（n=22,DM group）,and control group（n=20,C group）.DM group were randomly divided into two subgroups：DMl,DM2,and C group into Cl,C2.The group of DM2 and C2 were treated with Tongxinluo capsule for 5 weeks whereas DMl and C1 were given normal saline.The body weight and the blood sugar in all rats were measured before and after the treatment.The right kidneys of all the rats were removed and weighed,Pal-lmRNA expression in renal codex were measured by RT-PCR and agarose-gel electrophoresis densitometer.Results The levels of blood sugar and kidney-weight/body-weight,and PAI-1 mRNA expression were increased in diabetic rats compared with that in control group.The PAI-1 mRNA expression in renal cortex in DM2 was lower than that in DMl but higher than that in Cl.There were significant difference in other parameters between DMl and DM2,C1 and C2.Conclusion The expression of PAI-1 mRNA in renal cortex of diabetic rats was upregulated and PAI-1 might be play an important role in the occurrence and development of diabetic nephropathy.Tongxinluo capsule could suppress the increased expression of PAI-1 in renal cortex of diabetic rats.