银杏酮酯对再灌注损伤心肌内分泌因子的调节作用

目的观察银杏酮酯（Ginkgo bilobaextract50,GBE50）对心肌内分泌因子等的影响,探讨其抗心肌缺血再灌注（ischemiareperfusion,I/R）损伤的作用。方法 SD大鼠65只,随机分为6组：模型组、GBE50低剂量组、GBE50中剂量组、GBE50高剂量组、丹参组和地尔硫卓组。用Krebs-Henseleit（K-H）液或加药K-H液进行离体心脏Langendorff灌流,各组均平衡灌注15min,停灌造成全心缺血30min,再复灌40min。观察药物对冠脉流量、心肌超微结构、心肌组织血管紧张素Ⅱ（AngⅡ）、心房钠尿肽（ANP）含量的影响。结果与模型组比较,中、高剂量GBE50组冠脉流量的恢复率显著提高（P〈0.05）,心肌超微结构的损伤减轻。GBE50可明显降低心肌AngⅡ、ANP含量（P〈0.05）,部分提高ANP/AngⅡ比值。结论 GBE50对心肌缺血再灌注损伤具有保护作用,其作用机制可能与减少心肌中AngⅡ、ANP的产生有关。

Regulation Effect of Ginkgo Biloba Extract on Cardiac Endocrine Factors during Reperfuion Injury

Objective To observe the effect of Ginkgo biloba extract 50（GBE50）against myocardial ischemia-reperfusion injury（MIRI）,and approach its influence on cardiac endocrine factors preliminarily.Methods Sixty-five Sprague-Dawley rats were randomly divided into six groups：Control group,GBE50 group（low-,medium-and high-dose group）,Radix salviae miltiorrhizae（SM）group,and diltiazem group.After the heart was given Langendorff perfusion with Krebs-Henseleit（K-H）juice or K-H juice with drug for 15 min,the perfusion was stopped for 30 min to induce global ischemia,then it was reperfused for 40 min.Coronary flow,the content of angiotensin Ⅱ（AngⅡ）,and atrial natriuretic peptide（ANP）in myocardium were measured.The ultramicrostructure of myocardium were observed.Results Compared with control group,the recovery rate of coronary flow in medium-and high-dose GBE50 group was increased（P〈0.05）and the myocardium damage was alleviatived.The content of AngⅡ and ANP were significantly decreased in GBE50 groups（P〈0.05）,the ANP/AngⅡ ratio was raised partly.Conclusion GBE50 could play the role of cardioprotection against myocardial ischemia-reperfusion injury,which might be related to reducing AngⅡ and ANP in myocardium.