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| 抗人结肠癌相关抗原单克隆抗体的制备及初步研究 | |
| 引用本文： | 宋玉,刘晓燕,张萍,吴强,徐振山,宋礼华.抗人结肠癌相关抗原单克隆抗体的制备及初步研究[J].中国免疫学杂志,2006,22(4):353-355,359. |
| 作者姓名： | 宋玉 刘晓燕 张萍 吴强 徐振山 宋礼华 |
| 作者单位： | 1. 安徽大学生命科学院,合肥,230039 2. 安徽医科大学生物化学与分子生物学教研室,合肥,230032 3. 安徽医科大学病理学教研室,合肥,230032 4. 安徽安科生物工程(集团)股份有限公司,合肥,230088 |
| 摘 要： | 目的：制备抗人结肠癌相关抗原的单克隆抗体4D10，运用组织芯片技术研究其与人结肠癌组织反应的情况。方汪：用人结肠癌细胞株LOVO免疫小鼠，用间接细胞ELISA和免疫组化的方法筛选抗结肠癌相关抗原的单抗。结果：获得一株能够稳定分泌抗人结肠癌相关抗原的杂交瘤细胞株。4D10能够与不同分期的结肠癌组织切片反应，且与正常组织和其他癌组织几乎不反应。结论：4D10对结肠癌的病理分期及预后结果都具有一定的临床参考价值。 |
| 关 键 词： | 单克隆抗体 结肠癌 组织芯片 |
| 文章编号： | 1000-484X（2006）04-0353-04 |
| 收稿时间： | 2005-09-25 |
| 修稿时间： | 2005-09-252006-01-18 |
Preparation of monoclonal antibody against human colorectal cancer and its application in tumor research |
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| SONG Yu,LIU Xiao-Yan,ZHANG Ping,WU Qiang,XU Zhen-Shan,SONG Li-Hua.Preparation of monoclonal antibody against human colorectal cancer and its application in tumor research[J].Chinese Journal of Immunology,2006,22(4):353-355,359. | |
| Authors: | SONG Yu LIU Xiao-Yan ZHANG Ping WU Qiang XU Zhen-Shan SONG Li-Hua |
| Institution: | College of Life Science,Anhui University,Hefei 230088, China |
| Abstract: | Objective:To produce the mAb which specifically reacting with colorectal cancer confirmed by the tissue microarray technology.Methods:The mice were immunize with LOVO, and the mAb were screened by indirect cell ELISA and immunohistochemistry.Results:A McAb was obtained which can react to the colon cancer, but very little or not to normal tissue and other caner tissues.Conclusion:A McAb which can react to the colon cancer have been obtained,and the McAb can be useful on clinic for diagnosis and prognosis of colorectal cancer. |
| Keywords: | Monoclonal antibody Colorectal cancer Tissue microarray |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |