Effects of resistant starch RS4 on magnesium and iron absorption in Wistar rats

The effect of resistant starch RS4 on apparent absorption of magnesium and iron was studied in Wistar rats. The rats (4 groups male n=12 and 4 groups female n=12) were fed for 4 weeks diets: control with wheat starch (K) and 3 diets with modified resistant starches (S1, S2, S3). After an adaptation period (14 d), rats were transferred to metabolic cages. Dietary intake and faeces were monitored for 3 days. Mg and Fe levels were assessed in diets and feces by atomic absorption spectrometry. Apparent absorption of minerals was calculated as mineral intake minus fecal excretion and expressed as persentage of intake. Our results confirmed that Mg apparent absorption in female rats fed diet with resistant starch S2 was significantly increased (+37%) compared with the control group.