新生儿缺氧缺血性脑病红细胞免疫黏附功能和T细胞活性的检测

①目的 探讨缺氧缺血性脑病（ＨＩＥ）病儿红细胞和Ｔ细胞免疫状态及其与临床相关性。②方法 应用酵母菌花环法、间接免疫荧光法检测ＨＩＥ病儿红细胞免疫附功能、Ｔ细胞亚群和Ｔ细胞白细胞介素－２受体（ＩＬ－２Ｒ）表达。③结果 ＨＩＥ组急性期红细胞Ｃ３ｂ受体花环率（Ｅ－Ｃ３ｂＲＲ）、ＣＤ４＾＋细胞、ＣＤ４＾＋／ＣＤ８＾＋细胞比值和Ｔ细胞ＩＬ－２Ｒ表达率明显低于对照组（ｔ＝４．２９～４．７５，Ｐ〈０．０１），而

THE STUDY OF RED CELL IMMUNE ADHERENEC FUNCTION AND T CELL IMMUNE ACTIVITY IN NEWBORN INFANT WITH HYPOXIC ISCHEMIC ENCEPHALOPATHY

Objective To study the changes of immune status of red cell and T cell and the relationship between the changes of immune status and patient's condition in hypoxic ischemic encephalopathy (HIE). Methods Red cell immune adherence function, T cell subsets and expression of IL 2R on T cell in peripheral blood were detected by erythrocyte saccharomycete rosette and indirect immune fluoresence staining. Results During the acute stage of HIE, CD + 4 cell level, ratio of CD + 4/CD + 8 cell, E C 3b RR level, and expression rates of IL 2R on T cell in HIE groups were all lower than that of normal control group (t=4.29-4.75,P<0.01). CD + 8 cell level in HIE groups were higher than that of normal control group (t= 3.35 ,P<0.01). These changes became more significant with exacerbation of the illness. E C 3b RR level and expression rates of IL 2R on T cell in recovery period of HIE were still lower than that of normal control group(t=2.55,2.46,P<0.05) . Positive correlation was observed between E C 3b RR and CD + 4 cell, E C 3b RR and ratio of CD + 4/CD + 8 cell, as well as E C 3b RR and expression rates of IL 2R on T cell . Negative correlation was found between E C 3b RR and CD + 8 cell . Conclusion Red cell immune and T cell immune function were low in newborn infants with HIE and significant correlation was obsverved between them. These changes was closely related to the degree of HIE and development of disease.