基于人参皂苷含量的西洋参等级规格标准研究

目的：分析不同产地、不同等级规格西洋参中人参皂苷的含量,评价西洋参的化学信息,为其行业标准的制定提供实验依据。方法：采用UPLC测定主要活性成分人参皂苷Rg1、Re、Rb1、Rb2、Rb3、Rc、Rd、Rg2含量,用SPSS16.0软件进行统计分析。结果：建立了不同产地西洋参样品人参皂苷分布特征图谱;西洋参中Rg1、Re、Rb1的含量之和符合《中国药典》标准;方差分析表明产地、长度对同一个规格的西洋参中人参皂苷的含量影响具有统计学意义（P〈0.01）。结论：由于西洋参中人参皂苷含量受产地、长度、重量等多因素影响,在等级规格评价中,符合《中国药典》标准基础上不宜采用人参皂苷含量为指标进行等级规格划分。

Study on Grade Specification of American ginseng Based on the Content of Ginsenosides

Objective： To analyze the content of ginsenosides in American ginseng,and provide the scientific foundation for the quality control and standard establishment of American ginseng. Methods： The SPSS16. 0 software was used to analyze the data of eight compounds（ginsenosides Rg1,Re,Rb1,Rb2,Rb3,Rc,Rd and Rg2）determined by means of reversed-phase ultra-performance liquid chromatography（UPLC）. Results：The ginsenosides chromatogram has been established. The sum of three compounds（ginsenosides Rg1,Re,Rb1）content in American ginseng better meets the national standard of Chinese Pharmacopoeia. Two-way ANOVA analysis showed that both length and production regions have significant effects on the contents of ginsenosides. Conclusion： The contents of ginsenosides are influenced by many factors. It is not appropriate to establish the commercial specification of American ginseng based on the content of ginsenosides.