观察TRPV1受体在电针调节小鼠空肠运动中作用的实验研究

目的观察电针香草酸瞬时受体亚型1（transient receptor potential vanilloid 1，或vanilloid receptor 1，TRPV1）基因敲除小鼠的肠运动变化，初步探讨TRPV1受体在针刺调节肠运动过程中的作用。方法正常野生CL57BL/6小鼠和TRPV1基因敲除小鼠分别为B6组和TRPV1组，各15只。使用JL-F数显式光热测痛仪与ALMEMO2450机械测定仪检测2组小鼠光热痛阈值及机械痛阈值，比较2组小鼠之间痛阈值的差异。同时利用自制肠测压头置入空肠，监测空肠内压，待肠压力稳定采用2 mA、2/15Hz的刺激量电针曲池、上巨虚、天枢及大肠俞4穴，观察电针腧穴前后肠内压值的变化，并记录肠内压值变化波形图。结果（1）TRPV1组小鼠光热痛和机械痛较B6组小鼠明显迟钝，差异有统计学意义（P〈0.01）。（2）肠内压观察实验，针刺曲池、上巨虚穴，两组小鼠肠运动变化均不明显（P〉0.05）。（3）针刺TRPV1组小鼠天枢、大肠俞腧穴可引起肠运动变化，以抑制肠运动为主，其变化程度与B6组小鼠相当。结论生理状态下，TRPV1可介导光热及机械刺激，但TRPV1介导的针刺效应较为复杂，提示TRPV1受体可能为介导针刺调节肠运动因素之一。

Roles of TRPV1 Receptor in Electroacupuncture mental Study

Objective To observe the intestinal movement of transient receptor potential vanilloid 1 or vanilloid receptor 1 （TRPV1） knockout mice after stimulated by electroacupuncture （EA）, and to primarily explore the roles of TRPV1 receptor in the jejunal motility regulated by acupuncture. Methods Normal wild-type CL57BL/6 and TRPV1 gene knockout mice were recruited in two groups, the B6 group and the TRPV1 group, 15 in each group. The thermal threshold and the mechanical pain threshold were respectively detected using JL-F digital photo thermal analyzer instrument and ALMEMO2450 machine. The difference between the two thresholds were compared. Meanwhile, a self-made pressure head was placed in the jejunum. The internal pressure was monitored. When the pressure was stable, 2 mA 2/15 Hz EA at Quchi （Llll）, Tianshu （ST25）, Shangjuxu （ST37）, and Dachangshu （BL25） to observe the chan- ges of intestinal pressure value between before and after EA. The curve of internal pressures was recorded. Results （1） The stimulation of light/heat and mechanical stimulation were obviously slowed in the TRPV1 group than in the B6 group （P 〈0.01 ）. （2） In the intestinal pressure observation experiment, the bowel movement was not obviously seen in the two groups when acupunctured at Quchi （LI11） and Shangjuxu （ST37） （P 〉0.05）. （3） Acupuncture at Tianshu （ST25） and Dachangshu （BL25） of TRPV1 knockout mice could lead to intestinal movement （P 〈0.05）, mainly inhibited bowel movement. The changing degree was equivalent to that of B6 mice. Conclusions Under the physiological condition, TRPVl might be mediated by thermal and mechanical stimulation. But TRPV1 mediated acupuncture effect was quite complex, indicating TRPV1 mice might be one of intestinal movement mediating factors.