ALTERATION OF G1-CYCLINS (D1 AND E) IN TRANSITIONAL CELL CARCINOMA OF HUMAN URINARY BLADDER WITH INFECTION OF HPV-18

Human papillomaviruses (HPVs) are small double- stranded DNA viruses of more than 100 types which have been completely cloned and characterized1, 2]. They are related to numerous human tumors including cervical carcinoma in female. In the urinary tract, the relationship of HPV and transitional cell carcinoma (TCC) of urinary bladder is controversial as the reported detection of HPV DNA in the malignant tumors varied over a wide range (0-83.3%)3-7]. This variation in detection may depe…

Alteration of G1-cyclins (D1 and E) in transitional cell carcinoma of human urinary bladder with infection of HPV-18

Objective: This study was designed to investigate differential pattern of G1-cyclins (D1 and E) in transitional cell carcinoma (TCC) of human urinary bladder with or without human papillomavirus-18 (HPV-18) infection. Methods: Immunohistochemistry method was used in the detection of the expression of G1-cyclins in 57 cases of TCC (7 normal bladders as control), and HPV-18 DNA was found in 29 cases by polymerases chain reaction (PCR). Results: Cyclin D1 expression was found in 41 of 57 (71.93%) TCCs and it was reverse associated with HPV (x2=8.21, P<0.05). And cyclin D1 expression was found in 16 of 29 (55.17%) in HPV-18 infection group and 25 of 28 (89.29%) in non-HPV infection group. Cyclin E expression was detected in 36 of 57 (63.16%) and the association between the cyclin E expression and HPV infection was not found (x2=0.52, P>0.05). Cyclin E expression was found in 17 of 29 (56.82%) in HPV-18 infection group and 19 of 28 (67.86%) in non-HPV infection group. There was obvious difference in the cyclin D1 and cyclin E expression between the TCC and normal tissue (x2=7.46, P<0.05; x2=7.45, P<0.05, respectively). Conclusion: These data demonstrated that HPV infection altered the control of G1 cell cycle. And changes of G1 cell cycle regulatory proteins, either by interaction of cellular protein with viral oncoproteins or by changes in the cellular proteins themselves, may be critical for carcinogenesis of TCC of urinary bladder.