CHROMOSOME 3 MAY HARBOR MULTIPLE TUMOR SUPPRESSOR GENES ASSOCIATED WITH PRIMARY GLIOBLASTOMA MULTIFORME

Glioblastoma multiforme (GBM), the most malignant type of glioma, is the most common primary brainneoplasm. Although comprehensive therapeutic measures are applied, the prognosis of GBM remains dismal with a median post-treatment survival of less than one year.Modern molecular genetics has demonstrated thatabnormal alterations of tumor suppressor genes (TSGs) and oncogenes are the major mechanisms responsible for the initiation and progression of this malignant tumor.Identifying of related…

Chromosome 3 may harbor multiple tumor suppressor genes associated with primary glioblastoma multiforme

Objective: To investigate whether deletion ofchromosome 3 is involved in the carcinogenesis of primary glioblastoma multiforme (GBM) and to localize the possible common deletion region in the aforementionedchromosome. Methods: PCR based microsatellite polymorphism analyses were performed to detect loss of heterozygosity (LOH). Twenty-three loci on chromosome were examined in 20 cases of GBM. Fluorescence-labeled primers and Perkin Elmer 377 DNA Sequencer wereapplied. Results: 50% informative cases of GBM displayed LOH on chromosome 3. 50% of informative casesdisplayed LOH on 3q and 35% on 3p. 25.6% ofinformative loci showed LOH in our series, in whichfrequent LOH were observed in the chromosomal regionfrom loci D3S1614 (42.9%) to D3S1565 (35.3%) on 3q24-27 and at loci D3S1569 (35.3%) on 3q22-23 and D3S1289(33.3%) on 3p14.1-14.3. Conclusion: Loss of geneticmaterial on chromosome 3 may play an important part ithe tumorigenesis of GBM. The chromosomal regionsfrom loci D3S1614 to D3S1565 on 3q24-27 and at loci D3S1569 on 3q22-23 and D3S1289 on 3p14.1-14.3 are potential sites for novel tumor suppressor genes associated with GBM.