| POSSIBLE REASONS FOR TP53 ACCUMULATION IN NASO-PHARYNGEAL CARCINOMA USING ATLAS HUMAN CANCER cDNA EXPRESSION ARRAY |
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| 作者姓名： | 李虹 韩为农 张玲 冯湘玲 姚开泰 |
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| 作者单位： | LI Hong HAN Wei-nong，ZHANG Ling FENG Xiang-ling YAO Kai-tai Key Laboratory of Carcinogenesis of Ministry of Public Health，Cancer Research Institute，Xiangya Medical School，Central Southern University，Changsha 410078 |
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| 基金项目： | This work was supported by the Key Project of National Natural Science Foundation of China (No. 39730200)，the American CMB (96-655). |
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| 摘 要： | The incidence of nasopharyngeal carcinoma (NPC) in southern China and southeastern Asia is the highest in the world. The etiology of NPC is very complicated and has been associated with Epstein-Barr virus (EBV), chemical carcinogens and genetic susceptibility1]. Indeed, multi-factors may finally lead to the alterations of gene expression pattern of NPC. Therefore, to explore the changes of gene expression will provide direct evidence to the underlying mechanism of NPC pathogenesis. T…
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| 收稿时间： | 12 November 2001 |
| 修稿时间： | 22 January 2002 |
Possible reasons for TP53 accumulation in nasopharyngeal carcinoma using atlas human cancer cDNA expression array |
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| Hong Li,Wei-nong Han,Ling Zhang,Xiang-ling Feng,Kai-tai Yao.POSSIBLE REASONS FOR TP53 ACCUMULATION IN NASO-PHARYNGEAL CARCINOMA USING ATLAS HUMAN CANCER cDNA EXPRESSION ARRAY[J].Chinese Journal of Cancer Research,2002,14(1):28-32. |
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| Authors: | Hong Li Wei-nong Han Ling Zhang Xiang-ling Feng Kai-tai Yao |
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| Institution: | (1) Key Laboratory of Carcinogenesis of Ministry of Public Health, Cancer Research Institute, Xiangya Medical School, Central Southern University, 410078 Changsha |
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| Abstract: | Objective: To compare gene expression profiles of nasopharyngeal carcinoma (NPC) tissue with that of control tissue by cDNA Array and to discuss possible reasons of TP53 accumulation in NPC tissue. Methods: (1) hybridization of Atlas Human Cancer cDNA Expression Array 7742-1; (2) analysis of Atlas Arrays using Atlasimage 1.01a; (3) verification of results of array by RT-PCR; (4) verification of protein expression alterations by immuno- histochemistry. Results: (1) Of 588 tumor-related genes, 134 genes were upregulated, 88 downregulated; (2) Of 32 TP53-regulated genes, 13 genes were shown differential expression, 11 upregulated, 2 downregulated; (3) ATM and JNK2 were upregulated; (4) mRNA expression of ubiquitin-conjugating enzyme E2 (M74524) and ubiquitin- conjugating enzyme E2 (L22005) has no evident changes; Conclusion: (1) TP53 dysfunction exists in NPC tissues; (2) ATM and JNK might be the important causes of TP53 accumulation. |
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| Keywords: | cDNA Array Nasopharyngeal carcinoma TP53 |
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