| LD-RTPCR:A NEW METHOD FOR LABELLING TRACE cDNA MICROARRAY PROBE |
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| 作者姓名： | FAN Bao-xing 范保星 SUN Jing-fen 孙敬芬 LIANG Hao 梁好 WANG Sheng-qi 王升启 ZHOU Ping-kun 周平坤 WU De-chang 吴德昌 |
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| 作者单位： | Department of Molecular Toxicology，Beijing Institute of Radiation Medicine，Beijing 100850 |
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| 基金项目： | This work was supported by the National ?73?Key Basic Research Program of China (No.G1998051207). |
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| 摘 要： | cDNA microarrays are now being employed formRNA expression analysis in cancer cell lines1] orexcised surgical specimens2]. However, broaderapplication of cDNA microarrays is limited by theamount of RNA required: 50-200 mg of total RNA or 2-5 mg of poly A+ RNA. To broaden the use of cDNAmicroarrays, methods aiming at intensifying fluorescence signal3-5] have been tried and resulted in modest improvement. LD-RTPCR (SMARTTM PCR cDNA Synthesis Kit, Clontech) is a PCR-based …
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| 收稿时间： | 5 March 2002 |
| 修稿时间： | 26 July 2002 |
LD-RTPCR: A new method for labelling trace cDNA microarray probe |
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| Bao-xing Fan,Jing-fen Sun,Hao Liang,Sheng-qi Wang,Ping-kun Zhou,De-chang Wu.LD-RTPCR:A NEW METHOD FOR LABELLING TRACE cDNA MICROARRAY PROBE[J].Chinese Journal of Cancer Research,2002,14(3):179-182. |
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| Authors: | Bao-xing Fan Jing-fen Sun Hao Liang Sheng-qi Wang Ping-kun Zhou De-chang Wu |
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| Institution: | (1) Department of Molecular Toxicology, Beijing Institute of Radiation Medicine, 100850 Beijing |
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| Abstract: | |
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| Keywords: | LD-RTPCR RT cDNA Microarray Probe |
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