PREPARATION AND IDENTIFICATION OF MONOCLONAL ANTIBODIES AGAINST THE EXTRACELLULAR DOMAIN OF INTEGRIN a6 SUBUNIT-THE SPECIFIC LAMININ RECEPTOR

Integrin family belongs to the adhesive molecules of the cell surface. Integrins are transmembrane glycoproteins. Their receptor function requires the noncovalent linkage of an a and a b subunits. Integrin a6 can bind with either b1 or b4, and functions as a laminin receptor1]. The heterodimers?extracellular domain mediates the recognization and combination of the cell-cell and cell-extracellular matrix. The cytoplasmic domain interacts with cytoskeletal proteins and other signal transductio…

PREPARATION AND IDENTIFICATION OF MONOCLONAL ANTIBODIES AGAINST THE EXTRACELLULAR DOMAIN OF INTEGRIN a6 SUBUNIT-THE SPECIFIC LAMININ RECEPTOR

Objective: To prepare monoclonal antibody (McAb) against the Integrin a6 extracellular domain and identify its biological activities. Methods: Fusion-protein of integrin a6 extracellular domain (GST-IAGED) was expressed in E.coli. JM109 and used for immunizing BALB/C mice. The spleen cells from immunized mice were fused with SP2/0 cells and selectively cultured with HAT medium. ELISA and immunocytochemistry staining were used to select hybridomas. Results: One strain of hybridoma cells that secreted specific monoclonal antibody against integrin a6 extracellular domain was indentified. The immunoglobulin subclass of the McAb was IgG1. Conclusion: The McAb against the extracellular domain of integrin a6 was successfully prepared by using GST-IA6ED fusion protein expressed by E.Coli. And the McAb had positive reaction with human hepatocarcinoma cells-BEL-7402.