高效液相色谱法同时测定银花平感颗粒中10 种成分的含量

目的 建立高效液相色谱（HPLC）法同时测定银花平感颗粒中新绿原酸、绿原酸、隐绿原酸、葛根素、虎杖苷、异绿原酸A、异绿原酸C、白藜芦醇、甘草酸和大黄素10 种成分含量的方法。方法 采用ThermoAcclaim-C18 色谱柱（250 mm × 4.6 mm，5 μm），以乙腈-0.1% 磷酸水溶液为流动相进行梯度洗脱，流速：1.0 mL·min-1，采用327 nm（新绿原酸、绿原酸、隐绿原酸、虎杖苷、异绿原酸A、异绿原酸C、白藜芦醇）和252 nm（葛根素、甘草酸、大黄素）作为检测波长，柱温：30 ℃，进样量：10 μL。结果 银花平感颗粒中10 种成分分离度良好，新绿原酸、绿原酸、隐绿原酸、葛根素、虎杖苷、异绿原酸A、异绿原酸C、白藜芦醇、甘草酸和大黄素的线性范围分别为1.744～17.44、10.75～107.5、1.863～18.63、14.62～146.2、4.784～47.84、1.208～12.08、4.427～44.27、1.971～19.71、3.624～36.24 和4.142～41.42 μg·mL-1，线性关系良好（r ≥ 0.999 4）；平均加样回收率在99.17%～102.97% 之间（RSD ≤ 2.65%）。5 批样品中上述10 个成分含量范围依次分别为1.065～3.238、20.11～25.14、1.785～2.973、23.60～35.55、7.157～9.469、1.224～2.421、6.430～7.879、1.354～2.006、4.907～8.052、4.387～9.484 mg·g-1。结论 所建立的方法简单准确，可用于银花平感颗粒的质量控制与评价。

Simultaneous Determination of Ten Constituents in Yinhua Pinggan Granules by HPLC

Abstract： Objective To establish a HPLC method for simultaneous determination of neochlorogenic acid，chlorogenic acid， cryptochlorogenic acid， puerarin， polydatin， isochlorogenic acid A， isochlorogenic acid C，resveratrol， glycyrrhizic acid and emodin in Yinhua Pinggan Granules. Methods The analysis was performed onThermo Acclaim-C18 column （250 mm×4.6 mm，5 μm） by gradient elution of acetonitrile -0.1% phosphoric acidsolution at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 327 nm for detecting neochlorogenic acid，chlorogenic acid，cryptochlorogenic acid，polydatin，isochlorogenic acid A，isochlorogenic acid C and resveratrol，at 252 nm for detecting puerarin，glycyrrhizic acid and emodin. The column temperature was 30 ℃ and the injectionvolume was 10 μL. Results Good resolution of 10 constituents of Yinhua Pinggan Granules was obtained. The linearranges of neochlorogenic acid，chlorogenic acid，cryptochlorogenic acid，puerarin，polydatin，isochlorogenic acidA， isochlorogenic acid C， resveratrol， glycyrrhizic acid and emodin were 1.744-17.44， 10.75-107.5， 1.863- 18.63，14.62-146.2，4.784-47.84，1.208-12.08，4.427-44.27，1.971-19.71，3.624-36.24 and 4.142-41.42 μg·mL-1，respectively. The results showed good linear relationships （r ≥ 0.999 4）. The average recoveries ranged from 99.17%to 102.97%（RSD ≤ 2.65%）. The content ranges of ten constituents in five batches were 1.065-3.238，20.11-25.14，1.785-2.973， 23.60-35.55， 7.157-9.469， 1.224-2.421， 6.430-7.879， 1.354-2.006， 4.907-8.052， 4.387-9.484 mg·g-1， respectively. Conclusion This method is simple and accurate， which can be used for the qualitycontrol and evaluation of Yinhua Pinggan Granules.