| 家族性肌萎缩硬化症酵母研究模型的探索 |
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| 引用本文： | 张小华,王跃嗣,牛新华.家族性肌萎缩硬化症酵母研究模型的探索[J].滨州医学院学报,2009,32(2). |
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| 作者姓名： | 张小华 王跃嗣 牛新华 |
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| 作者单位： | 滨州医学院药学院生物技术教研室,烟台市,264003;滨州医学院药学院生物技术教研室,烟台市,264003;滨州医学院药学院生物技术教研室,烟台市,264003 |
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| 基金项目： | 国家自然科学基金，滨州医学院科技计划项目 |
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| 摘 要： | 目的 构建铜-锌超氧化物歧化酶(SOD1)基因缺失的酿酒酵母菌株, 为进一步以酵母为模型开展家族性肌萎缩硬化症发病机制及防治研究奠定基础.方法 运用LFH-PCR构建了带有遗传霉素抗性基因(KanMX4)的SOD1基因中断框,转化酿酒酵母BY5677,以添加G418的平板筛选出SOD1基因缺失菌株,并经PCR鉴定.结果 PCR扩增证实SOD1基因已被成功敲除;与野生菌株相比,SOD1基因缺失菌株生长缓慢.结论 成功构建了SOD1基因缺失的酿酒酵母菌株.
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| 关 键 词： | 家族性肌萎缩硬化症 酿酒酵母 SOD1 LFH-PCR |
Exploration of the yeast model of familial amyotrophic lateral sclerosis |
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| ZHANG Xiaohua,WANG Yuesi,NIU Xinhua.Exploration of the yeast model of familial amyotrophic lateral sclerosis[J].Journal of Binzhou Medical College,2009,32(2). |
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| Authors: | ZHANG Xiaohua WANG Yuesi NIU Xinhua |
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| Institution: | ZHANG Xiaohua WANG Yuesi NIU Xinhua Department of Biotechnology,Binzhou Medical University,Yantai 264003 |
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| Abstract: | Objective To construct the SOD1 gene deletion mutant of Saccharomyces cerevisiae and lay a foundation for studying the pathogenesis and treatment of familial amyotrophic lateral sclerosis based on the yeast model.Methods The SOD1:KanMX4 gene deletion fragment was constructed by long flanking homology LFH-PCR and transferred to yeast strain BY5677.The SOD1 gene deletion mutant was selected on plates containing G418 and confirmed by PCR.Results The PCR analysis showed that SOD1 gene in the yeast strain BY5677... |
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| Keywords: | SOD1 LFH-PCR |
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