中药炎克宁汤剂对大鼠输卵管阻塞性不孕模型Bcl-2及EGFR mRNA表达的影响

目的:研究中药炎克宁汤剂对输卵管阻塞性不孕模型大鼠输卵管上皮Bcl-2及EGFR mRNA表达的影响。方法:96只8w～12w Wistar雌性大鼠随机分为正常对照组、模型组、妇乐冲剂组、炎克宁低中高剂量组,每组16只。造模后20d开始给药,给药后15d、30d处死各组大鼠。采用原位杂交法检测输卵管上皮细胞Bcl-2及EGFR mRNA的表达。结果:正常对照组输卵管未见有Bcl-2 mRNA表达,妇乐冲剂组与模型组比较15d、30dBcl-2mRNA表达明显升高(P<0.05),EGFR mRNA表达明显下降(P<0.05);炎克宁低剂量组Bcl-2及EGFR mRNA表达与模型组比较无显著差异;炎克宁中剂量组Bcl-2及EGFR mRNA表达与妇乐冲剂组比较无显著差异,与模型组比较有显著性差异(P<0.05);炎克宁高剂量组Bcl-2及EGFR mRNA表达与其他各组比较均有显著差异(P<0.05)。结论:解毒除湿、化瘀止痛治疗输卵管阻塞性不孕模型大鼠,能抑制输卵管细胞凋亡,可能与调节Bcl-2和EGFR mRNA蛋白表达有关。

Effect of Yankening Decoction on the Expressions of Bcl-2 and EGFR mRNA in Oviduct Epithelium of Model Rats with Tubal Obstruction Infertility

objective: To observe the effect of Yankening decoction on the expression of Bcl-2 and EGFR mRNA in oviduct epithelium of model rats with tubal obstruction infertility. Methods: 96 rats were randomly divided into 6 groups: model group, normal group, Fule granule control group, Yankening decoction treatment group 1(low dosage),group 2(middle dosage), group 3(high dosage). The expressions of Bcl-2 and EGFR mRNA in oviduct epithelium were examined by in situ hybridization. Results: Compared with the model group, the expression of Bcl-2 mRNA was increased,the expression of EGFR mRNA was decreased significantly in Yankening decoction treatment group 1,2,3 and Fule granule control group, and there was significant difference between Yankening decoction treatment group 3 and Fule granule control group. Conclusions: Yankening decoction can reduce apoptosis of oviduct epithelium by regulating the expressions of Bcl-2 and EGFR mRNA.