高效液相色谱-二极管阵列检测器法测定馒头中的合成色素

目的建立一种前处理简单、灵敏度高、重现性好的馒头中合成色素的检测方法。方法采用高效液相色谱-二极管阵列检测(HPLC-DAD)法,样品用无水乙醇+氨水+水(7+2+1)溶液提取,Agilent HC-C18柱(250mm×4.6mm,5μm)液相色谱柱分离,乙酸铵缓冲溶液(0.02mol/L)-甲醇为流动相进行梯度洗脱,检测波长448nm。结果测定结果具有良好的线性和重复性(RSD:1.25%～2.85%),样品加标回收率为87.4%～90.3%,方法检出限为0.002g/kg。结论该方法具有简便、快速、准确等优点,可用于准确测定馒头中的合成色素。

Determination of synthetic color in steamed bread by high-performance liquid chromatography with diode array detection

Objective To establish a detecting method for determining the synthetic color in steamed bread with simple pretreatment,high sensitivity and good repeatability.Method By the method of high-performance liquid chromatography with diode array detection(HPLC-DAD),the samples was extracted by the solution of absolute ethyl alcohol,ammonia water and clear water(with the ratio of 7∶ 2∶ 1),and separated by liquid chromatographic column of Agilent HC-C18 column(250mm × 4.6mm,5μm).Conduct gradient elution for the mobile phase by ammonium acetate buffer solution-methanol.The detected wavelength was 448nm.Results The detected result showed good linearity and repeatability,and the relative standard deviation(RSD) were between 1.25%and 2.85%.The recovery rate of the samples was between 87.4% and 90.3%,and the detection limit of this method was 0.002g/kg.Conclusions The method was simple,rapid and accurate,and it was suitable for the determination of synthetic color in steamed bread.