ZBTB8A在胃癌中的表达及其临床意义

目的探讨胃癌中ZBTB8A在胃癌组织及细胞中的表达及其临床意义。方法采用实时荧光定量PCR（RT—PCR）检测ZBTBSAmRNA在正常胃黏膜细胞系GES-1与胃癌细胞系SGC7901和MGC803中的表达差异，以及在104例原发性胃癌组织及其对应癌旁组织和40例正常胃黏膜组织中的表达差异，并分析ZBTB8A表达与胃癌临床病理特征之间的关系。结果ZBTB8AmRNA在胃癌细胞系SGC7901、MGC803和正常胃黏膜细胞系GES-1中的表达量分别为0．00138±0．00015、0．00158±0．00021和0．00036±0．000055，其在SGC7901和MGC803细胞系中的表达量高于GES．1细胞系，差异有统计学意义（均P〈0．01）。ZBTB8AmRNA在胃癌组织、癌旁组织和正常胃黏膜组织中的表达量分别为0．0152±0．0126、0．0070±0．0061和0．0079±0．0036，其在胃癌组织中的表达量高于后两者（均P〈0．01），而癌旁组织与正常胃黏膜组织表达的差异无统计学意义（P〉0．05）。ZBTB8A表达与胃癌浸润深度、淋巴结转移、肿瘤分期及分化程度有关（均P〈0．05），但与患者年龄、性别、肿瘤大体分型和组织分型无关（均P〉0．05）。结论ZBTB8A可能是胃癌潜在的致癌因子，并参与胃腺癌细胞分化、浸润和转移等过程。

Expression of ZBTB8A in gastric cancer and its clinical significance

Objective To investigate the expression of ZBTB8A （zinc finger and BTB domain containing 8A） in gastric cancer tissues and its clinical significance. Methods Level of ZBTBSA mRNA in human normal gastric cell line GES-1, human gastric cancer cell line SGC7901 and MGC803 was detected by real-time PCR. Levels of ZBTBSA mRNA and protein in cancer tissues, adjacent cancer tissues from 104 cases with primary gastric cancer and normal gastric mucosal tissues from 40 cases without malignant gastric diseases were detected by RT-PCR and immunohistochemistry, respectively. Association between ZBTB8A expression and clinicopathology was analyzed. Results ZBTBSA mRNA expressions in SGC7901, MGCS03 and GES-1 cells were 0.00138±0.00015, 0.00158±0.00021, 0.00036±0.000055, respectively, and differences among SGC7901, MGCS03 and GES-1 were significant respectively （all P〈0.05）. ZBTB8A mRNA expression was significantly up-regulated in cancer tissues as compared to adjacent cancer tissues and normal tissues （0.0152±0.0126 vs. 0.0070±0.0061 and 0.0079± 0.0036, all P〉0.05）, while no significant difference was found between adjacent cancer tissues and normal tissues（P〉0.05）. ZBTBSA expression was significantly associated with invasive depth, lymph node metastasis, tumor stage, and degree of adenocarcinoma differentiation （all P〈0.05）, but not with age, gender, histological type, gross type （all P〉0.05）. Conclusion ZBTB8A may be a potential carcinogenic factor in gastric carcinoma, and may also be involved in gastric adenocarcinoma cell differentiation, cancer invasion and metastasis.