血管内皮生长因子D在食管鳞癌组织中的表达及其临床意义

目的探讨血管内皮生长因子D（VEGF．D）在食管鳞状细胞癌中的表达及意义。方法收集中山大学附属第一医院2009年3月至2010年2月间收治的39例食管鳞癌及癌旁组织标本。用原位杂交法检测肿瘤组织及癌旁组织中VEGF．DmRNA的表达情况；用免疫组织化学法检测D2．40表达，计算D2—40标记的微淋巴管密度（MLVD）。分析VEGF．DmRNA表达与食管鳞癌临床病理资料及MLVD的关系。结果肿瘤组织中VEGF．DmRNA阳性表达率为56.4％（22／39）．明显高于癌旁组织[2．6％（1／39），P〈0．05]。有淋巴结转移者VEGF．DmRNA阳性表达率明显高于无淋巴结转移者E92．9％（13／14）比36．0％（9／25），P〈0．05]。VEGF．DmRNA阳性表达者MLVD明显高于阴性表达者（8．20±1．22比5.31±0．97，P〈0．01）。结论VEGF—D可能通过诱导淋巴管生成促进食管鳞癌淋巴转移。

Expression of vascular endothelial growth factor D in human esophageal squamous cellcarcinoma tissue and its significance

Objective To investigate the expression of vascular endothelial growth factor D （VEGF-D） in human esophageal squamous cell carcinoma （ESCC） and its significance. Methods The expression of VEGF-C mRNA in tumor tissues and non-cancer tissues from 39 ESCC patients in our hospital from March 2009 to February 2010 was detected by in situ hybridization （ISH） method. The expression of D2-40 was detected by immunohistochemistry, and microlymphatic vessel density （MLVD） was determined by lymphatic endothelial specific marker D2-40. The associations of VEGF-C mRNA expression with clinical data and MLVD were analyzed. Results Positive ISH VEGF-D mRNA was observed in tumor tissue samples of 22 cases （56.4%, 22/39） and non-cancer tissue sample of 1 case （2.6%, 1/39）, whose difference was statistically significant（P〈0.05）. The expression of VEGF-D mRNA in ESCC was significantly associated with lymph node metastasis[92.9%（13/14） vs. 36.0%（9/25）, P〈 0.05] and MLVI） [ （8.20±1.22） vs. （5.31±0.97）, P〈0.01], but not significantly associated with age, sex, pathological grade and depth of infiltration. Conclusion VEGF-D can promote lymphatic metastasis of ESSC by induction of lymphangiogenesis.