用HPLC法和氨基酸分析仪测定多维氨基酸片中18种氨基酸

目的：RP-HPLC法和氨基酸分析仪（amino acid analyzer，AAA）法测定氨基酸含量的比较。方法：采用Agilent高效液相色谱系统和日立835-50氨基酸分析仪测定多维氨基酸片中18种氨基酸的含量。RP-HPLC法采用C18柱和邻苯二甲醛柱前衍生化进行，AAA法采用离子交换色谱柱和茚三酮柱后衍生化进行。结果：HPLC法和AAA法的变异系数分别〈1．5％和3％，最小检测量分别为3pmol和30pmol。HPLC法的测定值与AAA的测定值有相关性。两种测定方法的平均相对偏差为5．74％（0．24％～9．60％）。结论：两种方法都可用于测定氨基酸，但测定结果存在差异。

Determination of 18 kinds of amino acids in multi-amino-acid tablets by RP-HPLC method and amino acid analyzer

Objective: To compare the methods of RP-HPLC and amino acid analyzer(AAA) for determining concentrations of amino acids.Methods: Eighteen kinds of amino acids in multi-amino-acid tablets were measured by an Agilent HPLC and Hitachi 835-50 amino acid analyzer.One C_(18) column was used for HPLC method with o-pathalaldehyde precolumn derivation.An ion exchange chromatography column was used for AAA method with ninhydrin postcolumn derivation.Results: The coefficients of variation of HPLC and AAA methods were less than 1.5% and 3.0%,respectively.The minimal detectable(limit) was 3 pmol for HPLC method and 30 pmol for AAA method.The results obtained from RP-HPLC were correlated to those of AAA method.The average relative standard deviation of the two methods was 5.74%(ranged from 0.24% to 9.60%).Conclusion: Both methods can be used to determine the concentrations of amino acids,but there is difference between the results of the two methods.