雷公藤、关木通、益母草对大鼠肾毒性的比较研究

目的:比较观察雷公藤、关木通、益母草对大鼠的肾毒性.方法:雷公藤、关木通、益母草给大鼠连续灌胃90天,观察动物的一般情况、尿常规、尿微量蛋白、肝肾功能、肾脏病理形态学改变.剩余1/3大鼠进行停药30天恢复期观察.结果:雷公藤、关木通、益母草三种药物都会造成不同程度的尿常规异常、尿微量蛋白异常和肾功能改变,停药后肾功能指标有的可复,有的不可恢复.雷公藤、关木通、益母草三种药物的大剂量都会造成不同程度的肾脏形态学改变,但损害部位有所不同.停药后,肾实质损伤多数为可逆性,间质纤维化难于恢复.结论:雷公藤、关木通、益母草一定剂量均可以造成不同性质、不同程度的大鼠实验性肾损伤,有必要对益母草"非马兜铃酸性"肾脏毒性作用机制进行深入的系统研究.

Experimental study of the rat renal toxicity of Tripterygium wilfordii, Caulis aristolochiae and Leonurus

To compare the renal toxicity of tripterygium wilfordii, Caulis and leonurus in rats. Methods: Tripterygium wilfordii, Caulis hocquartiae and leonurus were given to rats per oral. After 90 days successive administration, 2/3 of the total rats were sacrificed, and changes on the general states of health?urine routing?urine small proteins?liver function?renal function?renal pathomorphology were observed. The rest were experienced 30 days restoration period, and repeated the detection of the above-mentioned indexes to observe the conditions of restoration?secondary and lingering effects. Results: The high and low-dose of Tripterygium wilfordii, Caulis hocquartiae and leonurus all can cause different degree abnormality of urine routing, urine small proteins and the changes of renal functions. After recover of drug withdrawal, some parameter of renal functional could become normal, but the others were unrecoverable. Tripterygium wilfordii?Caulis hocquartiae and leonurus all could cause different degree change of renal morphology at high dose, but the injury region of kidney were not the same. The recovery degrees of renal damage were not the same, after the three drugs of above-mentioned withdrawal. Renal parenchyma injury was reversable, but the renal interstitial fibrosis were almost irreparable. Conclusion: Tripterygium wilfordii?Caulis hocquartiae and leonurus could cause different character, different degree rat experimental renal damage, so it is necessary to carry out extensive deepgoing and systematic research of the mechanism of "non-aristolochic acid" renal damage of Leonurus.