| PRF 及所含三因子对大鼠脂肪干细胞增殖和黏附的影响 |
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| 引用本文： | 高洁,王明国,李秀梅,杨帅,李雪. PRF 及所含三因子对大鼠脂肪干细胞增殖和黏附的影响[J]. 实用口腔医学杂志, 2016, 0(4): 462-466. DOI: 10.3969/j.issn.1001-3733.2016.04.003 |
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| 作者姓名： | 高洁 王明国 李秀梅 杨帅 李雪 |
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| 作者单位： | 1. 中国人民解放军第 401 医院口腔科, 青岛,266071;2. 山东大学附属济南市中心医院口腔科;3. 济南市人民政府机关门诊部口腔科;4. 潍坊医学院口腔医学院 |
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| 基金项目： | 山东省科技发展项目(编号:2014GSF11804) |
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| 摘 要： | 目的：研究富血小板纤维蛋白（PRF）及其所含三因子 TGF-β1、PDGF-AB、VEGF 分别对大鼠脂肪干细胞（ADSCs）增殖和黏附的影响。方法：将 ADSCs 培养在含有 PRF 膜和不同浓度的 PDFG-AB、TGF-β1、BEGF 中，细胞黏附实验检测培养2 h 后细胞黏附能力，CCK-8法检测培养1～7 d 细胞增殖。结果：黏附实验显示，PRF 组的黏附细胞数显著高于阴性对照组（P ＜0．05）；不同浓度的 PDGF-AB 组内黏附细胞数的差异无统计学意义（P ＞0．05）；不同浓度的 TGF-β1、VEGF 组内黏附细胞数的差异有统计学意义（P ＜0．05）。CCK-8实验表明，PRF 组的细胞增殖在各个时间点（1、3、5、7 d）显著高于阴性对照组（P ＜0．05）；不同浓度的 PDGF-AB、VEGF 组内细胞的增殖差异有显著性（P ＜0．05）；不同浓度的 TGF-β1组细胞增殖的差异无显著性（P ＞0．05）。结论：PRF 能提高 ADSCs 的增殖和黏附的能力。PDGF-AB 和 VEGF 可促进 ADSCs 的增殖；TGF-β1和 VEGF 均可促进 ADSCs 的黏附，并呈一定的量效正相关。
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| 关 键 词： | 富血小板纤维蛋白(PRF) 脂肪干细胞(ADSCs) 细胞增殖 细胞黏附 转化生长因子(TGF-β1) 血小板源性生长因子-AB(PDGF-AB) 血管内皮生长因子(BEGF) |
The effects of PRF and its three growth factors on the proliferation and adhesion of rat ADSCs |
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| GAO Jie;WANG Mingguo;LI Xiumei;YANG Shuai;LI Xue. The effects of PRF and its three growth factors on the proliferation and adhesion of rat ADSCs[J]. Journal of Practical Stomatology, 2016, 0(4): 462-466. DOI: 10.3969/j.issn.1001-3733.2016.04.003 |
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| Authors: | GAO Jie WANG Mingguo LI Xiumei YANG Shuai LI Xue |
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| Affiliation: | GAO Jie;WANG Mingguo;LI Xiumei;YANG Shuai;LI Xue;Department of Stomatology,the 401st Hospital of PLA;Department of Stomatology,Ji’nan Central Hospital;Department of Stomatology,Ji’nan Municipal People’s Government;Hospital of Stomatology,Weifang Medical University; |
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| Abstract: | Objective:To study the effects of PRF and recombinant hPDGF-AB,TGF-β1 and VEGF on the proliferation and adhe-sion of rat adipose tissue-derived stem cells(ADSCs)in vitro.Methods:ADSCs were cultured with PRF membrane and various do-ses of PDGF-AB,TGF-β1 and VEGF,cell adhesion was examined by adhesion assay after 2 culture,cell proliferation was examined by CCK-8 kit after 1 -7 d culture.Results:Cell adhesion assay showed that the adhesive numbers of rat ADSCs in PRF group were significantly higher than those in the negative group(P <0.05).The adhesive numbers of the ADSCs treated by PDGF-AB showed no significantly difference among different concentration groups(P >0.05).The adhesive numbers of the ADSCs treated by VEGF or TGF-β1 at different concentrations showed significant difference(P <0.05).CCK-8 kit assay showed that at different time points, the A values of ADSCs in PRF group were significantly higher than those of the negative control group(P <0.05).The A values of ADSCs in VEGF or PDGF-AB groups at different concentrations showed significant difference(P <0.05).The A values of rat AD-SCs in TGF-β1 group at different concentrations were lower than those in the negative control group(P <0.05).Conclusion:PRF as a combination of growth factors may stimulate the proliferation and adhesion of rat ADSCs in vitro.PDGF-AB and VEGF may stim-ulate the proliferation of rat ADSCs.TGF-β1 and VEGF may stimulate the adhesion of rat ADSCs in a dose-response manner to some degree. |
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| Keywords: | PRF ADSCs Proliferation Adhesion TGF-β1 PDGF-AB VEGF |
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