牙髓卟啉单胞菌脂多糖诱导小鼠成骨细胞表达白细胞介素-23

目的：探讨牙髓卟啉单胞菌（P．e）脂多糖（LPS）对小鼠成骨细胞 IL-23mRNA 和蛋白表达的影响，以及磷脂酰肌醇-3激酶（PI3K）信号通路是否参与了该过程。方法：采用 real-time PCR 和 Western blot 法检测 P．e LPS 诱导 MC3T3-E1细胞表达IL-23mRNA 和蛋白的情况，以及用 PI3K 信号通路抑制剂 LY294002预处理细胞后，IL-23mRNA 和蛋白表达的变化。结果：P．e LPS 刺激 MC3T3-E1细胞后，IL-23mRNA 的表达增加具有浓度依赖性和时间依赖性（P ＜0．05），IL-23蛋白的表达增加具有时间依赖性（P ＜0．05）；LY294002预处理细胞后，P．e LPS 诱导的 IL-23mRNA 和蛋白的表达均显著降低（P ＜0．05）。结论：P．e LPS 能诱导小鼠成骨细胞表达 IL-23mRNA 和蛋白，PI3K 信号通路可能参与了此过程。

Porhyromonas endodontalis liopolysaccharide regulates interleukin-23 expression in mouse osteoblasts

Objective:To investigate the effects of Porhyromonas endodontalis(P.e)liopolysaccharide(LPS)on the expression of IL-23mRNA and protein in mouse osteoblasts MC3T3-E1 and the role of phosphatidylinositol 3-Kinases (PI3K)signaling pathway in this process.Methods:MC3T3-E1 cells were treated with different concentrations of P.e LPS for different hours,or pretreated with LY294002,a special PI3K inhibitor.The IL-23 mRNA and protein expression levels were detected by real-time PCR and Western blot respectively.Results:The level of IL-23 mRNA increased in MC3T3-E1 cells with the increase of concentration and the treatment time of P.e LPS(P <0.05).The IL-23 protein expression was increased by P.e LPS in a time dependent manner(P <0.05).The mRNA and protein of IL-23 decreased(P <0.05)after pretreatment with LY294002.Conclusion:P.e LPS can induce the expression of IL-23 mRNA and protein in MC3T3-E1 cells,and the PI3K signaling pathway may play a part in this process.