幼鼠早期癫痫持续状态对海马结构损伤的远期影响

目的　探讨幼鼠早期癫痫持续状态 (status epilepticus,SE)对成鼠后海马结构损伤的远期影响。方法　健康生后 1 5～ 2 0 d Wistar幼鼠 48只 ,随机分为生理盐水对照组和氯化锂 -匹罗卡品腹腔注射诱导的 SE组 ,并持续追踪至成鼠阶段 ,应用常规病理及电镜观察海马结构的形态学改变 ,同时应用 Timm组织化学染色方法进行苔藓纤维发芽研究。结果　约 2 /3 SE幼鼠发育至成鼠阶段后 ,海马结构的神经元仍可发现变性和坏死性改变 ,以 CA1区、CA3区和齿状回为重。 Timm染色见 CA3区有苔藓纤维发芽现象。约 1 /3 SE幼鼠发育至成鼠后未见海马结构损伤性改变。结论　幼鼠早期 SE造成的海马结构损伤性改变可持续至成鼠阶段 ,但存在个体差异 ,可能与个体耐受性有关。

A Long Term Injuring Effects on Hippocampal Formation after Status Epilepticus in Early Immature Rats

Objective To study the long term injuring effects on hippocampal formation in early immature rats after status epilepticus (SE). Methods Healthy postnatal Wistar rat pups (n48) were divided into control group of normal saline and Lithium-pilocarpine-inducedstatusepilepticusmodelgrouprandomly,wecontinue tofollowup the stage of mature rats.Conventionalhistopathological method and electronmicroscopywere utilized to observe morphological changes in hippocampal formation. In addition, Timm histochemical technique was adopted to study mossy fiber sprouting. Results About 2/3 of rats in status epilepticu group could result in hippocampal neuronal degeneration and necrosis, especially in subfields CA1, CA3 and dentate gyrus. Mossy fiber sprouting could be found in the CA3 subfield.There were about 1/3 SE rats without injures in hippocampal formation. Conclusions The damage of hippocampal formation caused by status epilepticus during early immature rats can sustain to the period of mature rats; But individual differences may exist, which may be associated with the individual tolerance.