SNAP-25, a SNARE protein, inhibits two types of K channels in esophageal smooth muscle |
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Authors: | Ji Junzhi Salapatek Anne Marie F Lau Helena Wang Guotang Gaisano Herbert Y Diamant Nicholas E |
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Affiliation: | Department of Medicine, University of Toronto, Ontario, Canada. |
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Abstract: | BACKGROUND & AIMS: The plasma membrane-associated soluble N-ethylmaleimide-sensitive factors attachment protein receptors (SNAREs), synaptosome-associated protein of 25 kilodaltons (SNAP-25), and syntaxin 1A, have been found to physically interact with and functionally modify membrane-spanning ion channels. Studies were performed in cat esophageal body and lower esophageal sphincter (LES) smooth muscle to (1) show the presence of SNAP-25, and (2) determine whether SNAP-25 affects K+ channel activity. METHODS: Single circular muscle cells from the esophageal body and sphincter were studied. Cellular localization of SNAP-25 and K+ channel activity were assessed. RESULTS: SNAP-25 was found in the plasma membrane of all regions examined. Outward K+ currents in body circular muscle were mainly composed of large conductance Ca2+-activated channel currents (K(Ca), 40.1%) and delayed rectifier K+ channel currents (K(V), 54.2%). Microinjection of SNAP-25 into muscle cells caused a dose-dependent inhibition of both outward K+ currents, maximal 44% at 10(-8) mol/L. Cleavage of endogenous SNAP-25 by dialyzing botulinum neurotoxin A into the cell interior resulted in a 35% increase in outward currents. CONCLUSIONS: SNAP-25 protein is present in esophageal smooth muscle cells, and inhibits both K(V) and K(Ca) currents in circular muscle cells. The findings suggest a role for SNAP-25 in regulation of esophageal muscle cell excitability and contractility, and point to potential new targets for treatment of esophageal motor disorders. |
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Keywords: | 4-AP, 4aminopyridine BoNT/A, botulinum neurotoxin A DTX, dendrotoxin GST, glutathione S-transferase IbTX, iberiotoxin 1-V, current-voltage KCa, Ca2+-activated K+ channels Kv, delayed rectifier K+ channels SNAP-25, synaptosome-associated protein of 25 kilodaltons SNARE, soluble N-ethylmale-imide-sensitive factors attachment protein receptors t-SNARE, target membrane SNARE TEA, tetraethylammonium chloride |
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