| HPLC法测定盐酸布替萘芬凝胶剂的含量 |
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| 引用本文： | 刘燕,曹瑞国. HPLC法测定盐酸布替萘芬凝胶剂的含量[J]. 齐鲁药事, 2005, 24(5): 284-286 |
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| 作者姓名： | 刘燕 曹瑞国 |
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| 作者单位： | 1. 山东省生物药物研究院,济南,250108
2. 山东正大福瑞达制药有限公司,济南,250014 |
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| 摘 要： | 目的采用HPLC法测定盐酸布替萘芬凝胶剂的含量。方法采用WatersSymmetryC18(3.9mm×150mm,5μm)色谱柱,以甲醇-醋酸钠缓冲液(73∶27)为流动相,流速为1.0ml·min-1,检测波长为282nm,柱温为35℃。结果盐酸布替萘芬在5.194～519.4μg·ml-1范围内,线性关系良好;最低检出限0.39ng;平均回收率为99.72%,RSD=0.52%(n=6)。结论该方法结果准确,精密度和重现性好,可用于盐酸布替萘芬凝胶剂的质量控制。
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| 关 键 词： | 盐酸布替萘芬 高效液相色谱法 凝胶剂 含量测定 |
| 文章编号： | 1672-7738(2005)05-0284-03 |
Determination of Butenafine hydrochloride in gel by HPLC |
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| Liu Yan,Cao Rui-guo. Determination of Butenafine hydrochloride in gel by HPLC[J]. qilu pharmaceutical affairs, 2005, 24(5): 284-286 |
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| Authors: | Liu Yan Cao Rui-guo |
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| Affiliation: | Liu Yan,Cao Rui-guo1 |
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| Abstract: | Objective Butenafine hydrochloride Gel was determined by HPLC.Methods Waters Symmetry-C_18 (3.9mm×150mm,5μm)column was used with the mobile phase of methanol-sodium acetate buffer(73∶27).The flow rate was 1.0ml·min -1 .The detective wavelength was 282nm.The temperature of column was 35℃.Results There was a good linear relationship within the range of 5.194～519.4μg·ml -1 .The detection limit was 0.39 ng.The average recovery and relative standard deviation of the method were 99.72% and 0.52%(n=6)respectively.Conclusion The method is accurate,precise and reproducible for the quality control of Butenafine hydrochloride Gel. |
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| Keywords: | Butenafine hydrochloride HPLC gel determination |
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