Immunochemical Characterization of a Human B Lymphocyte Differentiation Antigen (p65) |
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| Authors: | E. W. ADES S. FERRONE C. M. BALCH |
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| Affiliation: | Cellular Immunobiology Unit, Tumor Institute, Departments of Microbiology and Surgery, Comprehensive Cancer Center, and Veterans Hospital, University of Alabama Medical Center, Birmingham, Alabama, and Scripps Clinic and Research Center, Department of Molecular Immunology, La Jolla. California, USA |
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| Abstract: | A human B-cell differentiation antigen (BDA-1) with a molecular weight of 65,000 was Identified by use of an anti-B-cell xenoantiserum. BDA-1 was isolated by immunoprecipitation of cell lysates from a B-lymphoblastoid cell line (SB) and from blood B lymphocytes (sIg+ER−) of four normal individuals. It was not detectable in cell membrane lysates from two T-lymphoblastoid cell lines (HSB-2 and MOLT-3) or from enriched normal T cells (sIg−ER+). BDA is a singlechain molecule since its migration in SDS-PAGE gels was not altered by heating the protein to 100 C or by treatment with 2-mercaptoetbanol. Immunodepletion experiments demonstrated that the antigen recognized by anti-BDA xenoantiserum has neither structural nor antigenic relationships with la-like antigen or β-microglobulin. |
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