香烟烟气提取物对NR8383细胞吞噬功能的影响

目的 探讨不同浓度香烟烟气提取物(CSE)对大鼠肺泡巨噬细胞(NR8383)吞噬功能的影响.方法 通过CCK-8法分析细胞比活力,Annexin-V FITC/PI双染法检测细胞凋亡以及CFSE荧光标记分析细胞的分裂增殖,来确定CSE对NR8383细胞的作用浓度和作用时间.收集经不同浓度CSE处理24 h的NR8383细胞,与FITC标记的大肠杆菌共同孵育2 h后,用流式细胞仪检测胞内荧光强度,分析CSE对细胞吞噬功能的影响.结果 NR8383细胞的吞噬功能随着CSE浓度的变化先增强后降低.单独用CSE处理NR8383细胞,在100μg/ml CSE作用下,巨噬细胞的吞噬功能与正常对照组相比提高0.5倍,此时的细胞比活力最高.在CSE和LPS共同作用下,CSE的浓度为100μg/ml时,NR8383细胞的吞噬功能提高到正常水平的3倍.而当CSE浓度达到200μg/ml时,NR8383细胞的吞噬功能受到损伤,细胞凋亡率为54.1%.结论 CSE可以影响NR8383细胞的吞噬功能,在较低浓度时可能激活巨噬细胞的吞噬功能,但较高浓度时可能对巨噬细胞的吞噬功能造成损伤,CSE可能具有激活巨噬细胞吞噬功能的潜能.

Abstract：

Objective To investigate the phagocytosis function of cigarette smoke extracts (CSE)on the NR8383 cells. Methods The concentration of CSE and the optimal time was defined by cell counting kit-8 assay, Annexin V/PI cell apoptosis assay and CFSE cell proliferation assay. The cell was gained after exposed to the different concentration of CSE for 24 h and mixed with fluorescein-labeled Escherichia coli in 37℃ for 2 h. The fluorescence intensity was used to assay the phagocytosis function of NR8383 cells.Results The phagocytosis function of NR8383 cells may be changed by the concentration of CSE. In the concentration of 100 μg/ml, the phagocytosis function of NR8383 was enhanced 0.5 times than the normal cell when NR8383 cell was exposed to CSE, and the specific activity is the highest. When NR8383 cells were exposed to CSE and LPS, the phagocytosis function of NR8383 cells was enhanced 2 times than the normal cell. In the concentration of 200 μg/ml, the phagocytosis function of NR8383 cells was damaged, the rate of apoptosis is the 54. 1%. Conclusion Low concentration of CSE enhanced the phagocytosis function of NR8383 cells, but high concentration of CSE damaged the phagocytosis function of NR8383 cells. This study reveals a new role of CSE as an activator of macrophage function.

Effect of the phagocytosis function on NR8383 ceil exposed to cigarette smoke extracts

Objective To investigate the phagocytosis function of cigarette smoke extracts (CSE)on the NR8383 cells. Methods The concentration of CSE and the optimal time was defined by cell counting kit-8 assay, Annexin V/PI cell apoptosis assay and CFSE cell proliferation assay. The cell was gained after exposed to the different concentration of CSE for 24 h and mixed with fluorescein-labeled Escherichia coli in 37℃ for 2 h. The fluorescence intensity was used to assay the phagocytosis function of NR8383 cells.Results The phagocytosis function of NR8383 cells may be changed by the concentration of CSE. In the concentration of 100 μg/ml, the phagocytosis function of NR8383 was enhanced 0.5 times than the normal cell when NR8383 cell was exposed to CSE, and the specific activity is the highest. When NR8383 cells were exposed to CSE and LPS, the phagocytosis function of NR8383 cells was enhanced 2 times than the normal cell. In the concentration of 200 μg/ml, the phagocytosis function of NR8383 cells was damaged, the rate of apoptosis is the 54. 1%. Conclusion Low concentration of CSE enhanced the phagocytosis function of NR8383 cells, but high concentration of CSE damaged the phagocytosis function of NR8383 cells. This study reveals a new role of CSE as an activator of macrophage function.