自体外周血单个核细胞移植治疗下肢动脉缺血性疾病的研究

目的 分析59例下肢缺血性疾病患者进行自体外周血单个核细胞(MNC)移植的疗效及其与移植MNC数及CD34+细胞数的相关性,探索外周血MNC在下肢动脉缺血性疾病治疗中的优势.方法 对59例患者治疗前静息痛、冷感、间歇性跛行及组织损伤分别进行评分,而后动员并采集自体外周血MNC,于缺血下肢多位点等间距注射.评价治疗后第7天及4个月时的治疗效果,分析注入的MNC数及其中所含CD34+细胞数与疗效的相关性.结果 MNC移植后第7天及4个月时患者的下肢缺血均有不同程度的改善,CD34+细胞数量与疗效的相关系数为0.461(P=0.047),尼莫地平值=0.484+1.055×CD34+细胞数,MNC数量与疗效的相关系数为0.473(P=0.000),尼莫地平值=0.288+0.401×MNC数.结果 显示MNC数量比CD34+细胞数量与疗效的相关性更强.结论 注入的自体外周血MNC数较CD34+细胞数更能反映与临床疗效的相关性.

Abstract：

Objective To analyze the efficacy and its correlation with species of transplant cells of autologous mobilized peripheral blood(PB) mononucleated cells (MNCs) transplantation on 59 patients with lower limbs ischemia. Methods Fifty-nine patients were evaluated with symptoms scores and after that their PBMNCs were mobilized and collected and then injected into the ischemic area at equal distance. They effectiveness and scores were evaluated at 7th day and 4th month after therapy. The correlation of CD34 + cells and of MNCs with effectiveness was analysed respectively, and formula for c orrelations between them and effectiveness was calculated. Results After MNCs injection, the effectiveness was observed both at 7th day and 4th month . The correlation of MNCs with effectiveness was stronger than that of CD34 + cells ( the effectiveness was represented by nimodipine value), According to the formula of nimodipine value, the value of the latter =0.484 + 1. 055 × CD34 + cells number and the former = 0.288 + 0. 401 × MNCs number with a correlation coefficient of R =0. 461( P =0. 047 ) and R =0. 473 ( P =0. 000 ) respectively. Conclusion Autologous mobilized PBMNCs number is a better indicator for effectiveness than CD34 + cells number.

Analysis the advantage of autologous mobilized peripheral blood mononuclear cells transplantation on lower limbs ischemia disease

Objective To analyze the efficacy and its correlation with species of transplant cells of autologous mobilized peripheral blood(PB) mononucleated cells (MNCs) transplantation on 59 patients with lower limbs ischemia. Methods Fifty-nine patients were evaluated with symptoms scores and after that their PBMNCs were mobilized and collected and then injected into the ischemic area at equal distance. They effectiveness and scores were evaluated at 7th day and 4th month after therapy. The correlation of CD34 + cells and of MNCs with effectiveness was analysed respectively, and formula for c orrelations between them and effectiveness was calculated. Results After MNCs injection, the effectiveness was observed both at 7th day and 4th month . The correlation of MNCs with effectiveness was stronger than that of CD34 + cells ( the effectiveness was represented by nimodipine value), According to the formula of nimodipine value, the value of the latter =0.484 + 1. 055 × CD34 + cells number and the former = 0.288 + 0. 401 × MNCs number with a correlation coefficient of R =0. 461( P =0. 047 ) and R =0. 473 ( P =0. 000 ) respectively. Conclusion Autologous mobilized PBMNCs number is a better indicator for effectiveness than CD34 + cells number.