| 高效液相色谱-电化学检测法测定生物样品中单胺类神经递质及其代谢产物* |
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| 引用本文： | 杨佳凤,李娟,杨虹,潘桂湘,高秀梅. 高效液相色谱-电化学检测法测定生物样品中单胺类神经递质及其代谢产物*[J]. 天津中医药大学学报, 2007, 26(4): 192-195 |
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| 作者姓名： | 杨佳凤 李娟 杨虹 潘桂湘 高秀梅 |
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| 作者单位： | 教育部处部共建方剂学重点实验室,国家中药高新技术产业基地,天津现代中药研究中心,天津中医药大学,300193 |
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| 摘 要： | [目的]建立一种简单、快速、灵敏的测定生物样品中单胺类神经递质及其代谢产物的高效液相色谱方法.[方法]色谱柱采用Waters C18反相色谱柱(3.9 mm×150 mm,5 μm),流动相为水相(每500 mL中含辛烷磺酸钠1.25 g,磷酸二氢钾6.8 g,乙二胺四乙酸18 mg,pH值3.3)∶甲醇∶乙腈=78∶19∶3,流速为0.8 mL/min,柱温35 ℃;电化学检测器工作电极为玻璃碳电极,参比电极为银/氯化银(Ag/AgCl)电极,工作电压为0.7 V.[结果]5种物质保留时间分别为去甲肾上腺素3.6 min,5-羟吲哚乙酸4.5 min,高香草酸5.2 min,多巴胺6.2 min,5-羟色胺11.9 min;线性范围均为20-1 000 μg/L,最低检测限为4～8 μg/L.[结论]此方法测定生物样品中的单胺类神经递质及其代谢产物,简便,快速且灵敏度高.
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| 关 键 词： | 高效液相色谱-电化学检测法 单胺类神经递质 代谢物 |
| 文章编号： | 1673-9043（2007）04-0192-04 |
| 收稿时间： | 2007-05-02 |
| 修稿时间： | 2007-05-02 |
Determination of monoamine neurotransmitter and its metabolites by high performance liquid chromatography and electrochemical detection |
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| YANG Jia-feng,LI Juan,YANG Hong. Determination of monoamine neurotransmitter and its metabolites by high performance liquid chromatography and electrochemical detection[J]. Journal of Tianjin University of Traditonal Chinese Medicine, 2007, 26(4): 192-195 |
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| Authors: | YANG Jia-feng LI Juan YANG Hong |
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| Affiliation: | Key Laboratory of Pharmacology of TCM Formulae, Ministry of Education Research Center of TCM, Tianjin University of TCM, Tianjin 300193, China;Key Laboratory of Pharmacology of TCM Formulae, Ministry of Education Research Center of TCM, Tianjin University of TCM, Tianjin 300193, China;Key Laboratory of Pharmacology of TCM Formulae, Ministry of Education Research Center of TCM, Tianjin University of TCM, Tianjin 300193, China;Key Laboratory of Pharmacology of TCM Formulae, Ministry of Education Research Center of TCM, Tianjin University of TCM, Tianjin 300193, China;Key Laboratory of Pharmacology of TCM Formulae, Ministry of Education Research Center of TCM, Tianjin University of TCM, Tianjin 300193, China |
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| Abstract: | |
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| Keywords: | HPLC-ECD Monoamine Neurotransmitter Metabolites |
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