外周血淋巴细胞热应激蛋白72和73水平与煤矿粉尘暴露及慢性阻塞性肺病的关系

目的探讨煤矿工人外周血淋巴细胞热应激蛋白Hsp72和Hsp73在慢性阻塞性肺病(COPD)患者和煤矿工人中的表达及Hsp72和Hsp73与粉尘暴露的关系。方法选择山西某煤矿工人中男性COPD患者50例,按性别、年龄匹配该矿健康煤工对照50例。分别测定肺功能,用流式细胞仪测定各组外周血淋巴细胞在体外基础状态、受热应激及二氢二醇环氧苯并(a)芘(BPDE)染毒后细胞内Hsp72和Hsp73的表达水平。结果(1)煤工COPD组和健康煤工组外周血淋巴细胞Hsp72及Hsp73的基础状态与体外受热和染毒应激后的表达呈一致性趋势,差异无统计学意义(P＞0．05)。(2)煤工COPD组外周血淋巴细胞Hsp72(17．7±4．9)表达低于健康煤工组(22．6±10．0),差异有统计学意义(P＜0．01),而Hsp73水平(33．5±11．7)明显高于健康煤工组(19．6±5．9),差异有统计学意义(P＜0．01)。(3)外周血淋巴细胞Hsp72表达水平与累积呼吸性粉尘暴露量呈正相关(健康煤工组：r= 0．30,P＜0．05；煤工COPD组：r=0．45,P＜0．01),而Hsp73表达与累积呼吸性粉尘暴露量无明显关系。结论煤工COPD患者中应激诱导的外周血淋巴细胞Hsp72表达下调,Hsp72表达与机体耐受粉尘毒性的能力可能相关。

Relationship between dust exposure and chronic obstructive pulmonary diseases and heat shock protein 72 and 73 in lymphocytes among coal miners

OBJECTIVE: To assess the expression of Hsp72 and Hsp73 in chronic obstructive pulmonary disease (COPD) and to evaluate their roles in damage from coal dust exposure. METHODS: A case control study of 50 coal miners suffering from COPD and 50 healthy coal miners were selected from one coal mine. The levels of Hsp72 and Hsp73 in peripheral blood lymphocytes were determined by flow cytometry for all subjects. RESULTS: (1) The expression of basic Hsp72 of peripheral blood lymphocytes for patients and controls was not different from that inducible expressed Hsp72 by 42 degrees C heat stress or by BPDE exposure. (2) The expression of Hsp72 in COPD patients (17.7 +/- 4.9) was significantly lower than that in healthy coal miners (22.6 +/- 10.0) (P < 0.01). On the other hand, the expression of Hsp73 in COPD patients (33.5 +/- 11.7) was higher than that in healthy coal miners (19.6 +/- 5.9) (P < 0.01). (3) A-positive relationship between the expression of Hsp72 and cumulative inhaling coal dust exposure was observed. No relationship was found between Hsp73 and cumulative inhaling coal dust exposure. CONCLUSION: The decreased expressions of Hsp72 in peripheral blood lymphocytes of COPD coal miners.