下瘀血汤中大黄生熟互换对热结血瘀模型大鼠血管内皮功能及微循环的影响

目的 研究下瘀血汤中大黄生熟互换对热结血瘀模型大鼠血管内皮功能及微循环的影响。方法 采用ig 热性中药结合sc 盐酸肾上腺素的方法，复制热结血瘀大鼠模型。大鼠ig 热性中药28 d，第22 天起sc 盐酸肾上腺素，并ig 相应的药物。第29 天，眼眶取血检测检测血管内皮素（ET）、一氧化氮（NO）、前列环素（PGI₂）、血管性血友病因子（vWF）；股动脉取血，检测血液流变学各项指标。结果 含生大黄（复方A）和熟大黄（复方B）下瘀血汤均具有改善热结血瘀模型大鼠血液流变学、血管内皮细胞损伤和微循环的作用；与复方A 相比，复方B 各剂量组对血液流变学各指标、ET、NO、PGI₂、vWF 因子水平具有显著的改善作用（P<0.05、0.01）。结论 含熟大黄下瘀血汤的活血化瘀作用更强，可能是通过调节ET、NO、PGI₂、vWF 因子水平而实现的。

Effect of raw/stewed Rhubarb in Xiayuxue Decoction on vascular endothelial function and microcirculation of rats with heat accumulation of blood stasis

Objective To study the effect of raw/stewed Rhubarb in Xiayuxue Decoction on vascular endothelial function and microcirculation of rats with heat accumulation of blood stasis Methods Seventy SD rats were divided into seven groups. The rats in experimental groups were given heat drugs of traditional Chinese medicine for 28 d, then injected adrenaline hydrochloride for later 7 d, twice a day. At the same time, those experimental rats were given different doses of Xiayuxue Decoction (9 and 4.5 g·kg^-1) orally for 7 d. Then blood rheologic parameters such as ET, NO, PGI₂, and VWF in rats were detected. Results Xiayuxue Decoction A/B have improved vascular endothelial cell injury and the microcirculation of the rats with heat accumulation of blood stasis. Compared with Xiayuxue Decoction A, each dose of Xiayuxue Decoction B has improved the levels of ET, NO, PGI₂, and vWF factor significantly (P < 0.05, 0.01). The efficiency on promoting blood circulation to remove blood stasis of Xiayuxue Decoction B was definitely better than Xiayuxue Decoction A. Conclusion The mechanism of promoting blood circulation to remove blood stasis may be through regulating the levels of ET, NO, PGI₂, and VWF factor.