| Abstract: | Objective: In this study we examined the effect of GM-CSF in combination with IL-4 on the bone marrow derived human adult mesenchymal stem cells (hMSCs).
Method: The hMSCs were isolated and cultured with GM-CSF and IL-4 for one month, and then we isolated the single colony of transformed cells and characterized the phenotype of them by morphology, surface marker expression and in vivo tumorigenesis.
Result: After one month culture, the transformed mesenchymal cells exhibited the morphology and phenotype similar to tumor cells, causing multiple fast growing lung deposits when injected into immunodeficient mice.
Conclusion: Cytokines-driven malignant transformation of hMSCs may be a useful model for studying signaling pathways initiating malignant transformation of hMSC. |
