毛菊苣提取物对四氯化碳诱导大鼠肝纤维化的防治作用

目的 观察毛菊苣提取物抗慢性肝纤维化的作用。方法 将Wistar大鼠随机分为毛菊苣提取物低、中、高剂量（50、100、150 mg/kg）组，秋水仙碱（colchicine，0.5 mg/kg）组，模型组和正常组，ig给药。给药同时各组sc四氯化碳（CCl₄）造模，每周2次。于60 d后，检测大鼠血清丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST）、总蛋白（TP）、白蛋白（ALB）、肝组织丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH-Px）、羟脯氨酸（Hyp）及肝组织病理变化。结果 与模型组比较，毛菊苣提取物低、中、高剂量组均能明显降低肝纤维化大鼠血清中ALT、AST的含量及肝组织中MDA和Hyp的含量（P＜0.01），明显提高血清TP、ALB水平（P＜0.05、0.01）及肝组织GSH-Px活性（P＜0.01）；肝脏组织学检查表明，毛菊苣提取物可明显改善肝组织病理损伤程度，其作用呈一定的量效关系。结论 毛菊苣提取物对实验性慢性肝损伤具有保护肝细胞，减少肝损伤，抗肝纤维化作用。

Preventive and therapeutic effects of Cichorium glandulolsum extract on carbon tetrachloride-induced liver fibrosis in rats

Objective To investigate the effects of Cichorium glandulolsum extract (CGE) on liver fibrosis induced by chronic hepatic injury. Methods Wistar rats were randomizedly divided into six groups: CGE (50, 100, and 150 mg/kg) treatment groups, colchicine (Colc, 0.5 mg/kg) treatment group, model group, and normal control group. Rats were sc injected with carbon tetrachloride (CCl₄) two times a week to induce liver fibrosis as well as ig administration of CGE or Colc. After 60 d, the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total protein (TP), albumin (ALB) in serum and malondialdehyde (MDA), glutathione peroxidase (GSH-Px), hydroxyproline (Hyp) in liver tissue were determined, and the liver histological changes were investigated by pathological examination. Results Compared with the model group, CGE significantly reduced the levels of ALT and AST in serum (P < 0.01), and decreased MDA and Hyp levels in liver tissue (P < 0.01) while TP, ALB, and GSH-Px were significantly elevated (P < 0.05, 0.01). Pathological examination revealed that CGE had the therapeutical effect on the experimental liver fibrosis. Conclusion CGE has the better effects on protecting hepatocyte, relieving liver injury, and against liver fibrosis.