二甲基亚硝胺肝硬化大鼠血清元素含量变化及其意义

目的　探讨二甲基亚硝胺 (DMN)肝硬化大鼠血清元素含量变化与肝功能损伤的关系及其在肝纤维化形成与发展中的意义。方法　用 0 .5 %DMN 生理盐水 2ml/kg给大鼠腹腔注射 ,共 4周 12次 ,分别于造模第 2周与第 4周结束后获取大鼠肝组织与血清 ,作肝病理组织学、肝组织羟脯氨酸 (Hyp)含量、肝功能及血清元素含量测定。 结果　( 1)造模第 2周时大鼠的血清ALT活性和TBIL显著升高 ,为同期正常组的 1.7倍 (P <0 .0 1)和 7.2倍 (P <0 .0 5 ) ;造模第 4周时为同期正常大鼠的 3 .4倍 (P <0 .0 1)和 3 4.6倍 (P <0 .0 1) ;第 4周模型大鼠Alb含量显著低于同期正常大鼠(P <0 .0 1)。 ( 2 )造模第 2周时大鼠的肝组织Hyp含量是同期正常组的 1.8倍 (P >0 .0 5 ) ,第 4周时较同期正常组增高了 3 .8倍 (P <0 .0 1)。 ( 3 )造模第 2周时血清元素主要表现为Cu含量显著增高 (P <0 .0 5 ) ,Cu/Zn比值也显著升高 (P <0 .0 1) ;第 4周时模型大鼠的血清Fe含量显著增高 (P >0 .0 5 ) ,Zn含量显著降低 (P <0 .0 1) ;血清Se含量较第 2周时模型大鼠进一步下降 ;Cu/Zn比值显著升高 (P <0 .0 1) ;Zn/Fe比值、Se/Fe比值均显著降低 (P <0 .0 1)。 ( 4 )以第 4周正常组和模型大鼠血清元素含量与肝组织Hyp含量及肝功能各指标作相关分析 ,血清

Changes of serum metallic elements in dimethylnitrosamine-induced cirrhosis in rats and their significances

Objective To investigate the relevance of serum metallic elements and liver function in dimethylnitrosamine-induced cirrhosis in rats and the role of these metallic elements in the development of progression of liver fibrosis. Methods 0.5% dimethylnitrosamine normal saline 2ml/kg was injected intraperiloneally 3 times a week for 4 weeks.The rats were sacrificed at 2 and 4 weeks respectively, their liver tissues and sera were used for pathology, liver tissue hydroxyproline (Hyp) content,liver function and determination of serum elements.Results (1)ALT activity and total bilirubin increased 1.7 times and 7.2 times those of the normal rats after 2 weeks (P<0.01), (P<0.05)respectively, and 3.4 times and 34.6 times those of the normal rats after 4 weeks(Pall<0.01) . Serum Alb was significantly lower than that of the normal rats (P< 0.01). (2)Liver tissue Hyp content was 1.8 times that of the normal rats after 2 weeks (P>0.05), but 3.8 times after 4weeks. (3)Serum Cu(P<0.05)and Cu/Zn ratio(P<0.01) increasd significantly after 2 weeks whereas, serum Fe content increased significantly after 4 weeks(P<0.05), Zn content decreased significantly (P<0.01), serum Se content decreased continuously in comparison with that at 2 weeks, Cu/Zn ratio increased significantly(P<0.01), but Zn/Fe ratio and Se/Fe ratio decreasd significantly (P<0.01). (4)Correlation analysis revealed: Serum Zn was correlated negatively with liver tissue hydroxyproline and correlated positively with serum Alb; Serum Fe was correlated positively with serum TBA, Cu/Zn ratio was correlated positively with liver tissue Hyp conent, serum ALT, ALP, GGT were all positively correlated with TBA content, but negatively correlated with serum Alb. Serum Zn/Fe ratio was correlated positively with tissue Hyp content, serum ALT, ALP. GGT, TBIL and TBA.Conclusion Changes in serum metallic elements are the consequences of liver injury. They also enhance the formation of liver fibrosis and cirrhosis; Zn supplementation can promote the excretion of Fe and Cu and it might be of potential value in the prevention of liver fibrosis and cirrhosis.