| RASSF1A基因对肝癌细胞化疗药物敏感性的影响 |
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| 引用本文： | 芮理,薛万江,李鹏,王鹏,王志伟,李厚祥. RASSF1A基因对肝癌细胞化疗药物敏感性的影响[J]. 肿瘤防治研究, 2010, 37(4): 414-416. DOI: 10.3971/j.issn.1000-8578.2010.04.012 |
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| 作者姓名： | 芮理 薛万江 李鹏 王鹏 王志伟 李厚祥 |
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| 作者单位： | 226001江苏南通大学附属医院普外科 |
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| 基金项目： | 南通市社会发展科技计划资助项目，南通大学自然科学资助项目 |
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| 摘 要： | 目的探讨RASSF1A基因的表达对肝细胞肝癌化疗药物敏感性的影响。方法利用已构建成功的稳定表达野生型和突变型RASSF1A基因的肝癌细胞株QGY-7703，化疗药物Mitomycin、Adriamycin、Etoposide、5-Fluorouracilur、Cisplatin分别作用各细胞株后，比较生长抑制率、细胞周期及P53、P21、Bax、Caspase-3蛋白的表达水平。结果野生型RASSF1A的表达可提高Mitomycin诱导的肝癌细胞生长抑制率、凋亡发生率（P<0.05）和Caspase-3的活性，对P53、P21、Bax基因的蛋白表达水平没有影响。结论野生型RASSF1A基因可提高肝癌细胞对Mitomycin的敏感性。
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| 关 键 词： | RASSF1A 肝细胞癌 药物敏感性 凋亡 |
| 收稿时间： | 2008-12-03 |
| 修稿时间： | 2009-07-12 |
Effects of RASSF1A Expression on Chemosensitivity of Hepatocellular Carcinoma Cell |
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| RUI Li,XUE Wan-jiang,LI Peng,WANG Peng,WANG Zhi-wei,LI Hou-xiang. Effects of RASSF1A Expression on Chemosensitivity of Hepatocellular Carcinoma Cell[J]. Cancer Research on Prevention and Treatment, 2010, 37(4): 414-416. DOI: 10.3971/j.issn.1000-8578.2010.04.012 |
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| Authors: | RUI Li XUE Wan-jiang LI Peng WANG Peng WANG Zhi-wei LI Hou-xiang |
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| Affiliation: | Department of General Surgery, The Affiliated Hospital of Nantong University, Nantong 226001, China |
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| Abstract: | Objective To explore the effect of RASSF1A on the chemosensitivity of human hepatocellular carcinoma (HCC) cell line QGY-7703.Methods The QGY-7703 cells expressing RASSF1A gene (wild type or mutant) stably were used in this study. The cells were treated with the antitumor drugs including Mitomycin, Adriamycin, Etoposide, 5-Fluorouracilur and Cisplatin. Then the rate of cell growth inhibition, changes of cell cycle and expression levels of p53, p21, bax and caspase-3 were measured.Results Expression of wild-... |
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| Keywords: | RASSF1A RASSF1A Hepatocellular carcinoma Drug-sensitivity Apoptosis |
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