| 小鼠真核表达质粒pcDNA3.1(+)-FGFR1的构建及表达 |
| |
| 引用本文： | 郭峻莉,翁志宏,郑少江. 小鼠真核表达质粒pcDNA3.1(+)-FGFR1的构建及表达[J]. 海南医学院学报, 2010, 16(10): 1245-1247,1252 |
| |
| 作者姓名： | 郭峻莉 翁志宏 郑少江 |
| |
| 作者单位： | 1. 海南医学院海南省热带病重点实验室,海南,海口,571101
2. 华中科技大学同济医学院附属协和医院传染科,湖北,武汉,430022 |
| |
| 基金项目： | 国家自然科学基金，教育部新世纪优秀人才支持计划项目，教育部科学技术研究重点项目，海南省自然科学基金 |
| |
| 摘 要： | 目的:通过基因重组技术构建真核表达质粒pcDNA3.1(+)-FGFR1,并检测其在CHO细胞中的表达。方法:通过PCR扩增FGFR1的全段基因cDNA,应用基因工程技术将扩增的基因片段插入pcDNA3.1(+)真核表达质粒,经限制内切酶酶切分析及测序鉴定正确后,用脂质体包裹转染CHO细胞,Western blot检测FGFR1目的蛋白的表达。结果:经酶切和测序鉴定证实本实验构建的重组表达质粒正确,该质粒在体外转染CHO细胞后可表达FGFR1目的蛋白。结论:成功构建的pcDNA3.1(+)-FGFR1重组质粒能在体外表达FGFR1目的蛋白。
|
| 关 键 词： | 碱性成纤维细胞生长因子1型受体(FGFR1) 真核表达质粒 基因工程 |
Construction and expression of eukaryotic expression plasmid vector pcDNA3.1(+)-FG-FR1 in vitro |
| |
| GUO Jun-li,WENG Zhi-hong,ZHENG Shao-jiang. Construction and expression of eukaryotic expression plasmid vector pcDNA3.1(+)-FG-FR1 in vitro[J]. Journal of Hainan Medical College, 2010, 16(10): 1245-1247,1252 |
| |
| Authors: | GUO Jun-li WENG Zhi-hong ZHENG Shao-jiang |
| |
| Affiliation: | GUO Jun-li,WENG Zhi-hong,ZHENG Shao-jiang (1.Hainan Provincial Key Laboratory of Tropical Medicine,Hainan Medical College,Haikou 571101,China,2.Department of Infectious Diseases,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China) |
| |
| Abstract: | Objective: To construct a recombinant eukaryotic expression plasmid pcDNA3.1(+)-FGFR1,and detect its expression in the cell of CHO.Methods: The full-length gene of murine FGFR1 was amplificated by PCR,and then was inserted into the eukaryotic expression plasmid pcDNA3.1(+).After the recombinant plasmid was confirmed by restriction endonuclease analysed and sequencing test,it was transfected into eukaryotic cell CHO by lipofectin.The FGFR1 protein was explored by Western blot.Results: The constructed recombi... |
| |
| Keywords: | Fibroblast growth factor receptor-1(FGFR1) Eukaryotic expression plasmid Genetic engineering |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
