系数倍率分光光度法在三组分复方扑热息痛片含量测定中的应用

本文介绍了系数倍率分光光度法用于三组分体系测定的基本原理和实验方法,用该法在7520型分光光度计上测定复方扑热息痛片中阿斯匹林、扑热息痛和咖啡因的含量。本文借助于微处理机选择最佳测定波长,不经分离直接测定四个波长的吸收值,可同时分别测得三组分的含量。方法简便快速,其平均回收率;阿斯匹林为99.6±0.36%;扑热息痛为100.0+0.37%;咖啡因为100.6±0.74%(95%置信率)。

Quantitative analysis of a three-component mixture--compound tablets of acetaminophen using signal multiplier spectrophotometry

This paper provides a basic principle and experimental technique of signal multiplier spectrophotometry. Quantitative analysis of three component mixture can be done without sample separation. This method was investigated to assay the contents of aspirin, acetaminophen and caffeine in compound tablets of acetaminophen by using a manual spectrophotometer (UV 7520). The selection of optimal wavelength pairs is performed through the program with a microcomputer. The contents of the three components could be analyzed when the absorption values at 4 wavelengths were determined. The average recoveries for each component are 99.6±0.36%, 100.0±0.37%, and 100.6±0.74, respectively (95% confidence level). Linearity and precision of analysis are good and the method is easy to employ.