黄芪多糖对糖尿病大鼠血管内皮细胞的影响

探讨黄芪多糖对糖尿病大鼠血管内皮细胞损伤的治疗作用及其机理。 3 0只SD大鼠随机分成正常对照组、四氧嘧啶糖尿病组与糖尿病黄芪多糖 (APS)治疗组 (黄芪多糖 4 0 0mg/kg/d-1腹腔注射 ) ,5周后 ,处死动物取血测血糖浓度 ,血清胰岛素 ,超氧化物歧化酶 (SOD) ,丙二醛 (MDA) ,内皮素 (ET) ,一氧化氮 (NO) ,并应用形态定量的方法比较各组的心肌病理检查结果。结果 ,腹腔注射四氧嘧啶 ( 2 0 0mg/kg)后的SD大鼠血糖浓度显著升高 ,血清NO ,MDA ,SOD ,ET及胰岛素水平明显改变。APS治疗 5周后 ,血糖浓度 ,MDA和ET较未治疗组显著下降 ;而NO ,胰岛素和SOD的含量显著升高。切片光镜下观察 ,糖尿病大鼠心肌毛细血管数量减少 ,基底膜增厚 ,微血管与心肌纤维的比率显著降低 ,这些改变都可被APS改善。黄芪多糖可降低糖尿病大鼠血糖水平 ,对早期糖尿病大鼠内皮细胞具有良好的保护作用 ,这可能与其减轻氧自由基的损伤 ,影响NO ,ET的产生以及促进胰岛B细胞损伤的恢复有关

Effect of Astragalus Polysaccharide on Vascular Endothelial Cells of Diabetic Rats

Objective :To investigate the effect of Astragalus Polysaccharide(APS) on pathology of diabetic microvessel. Methods :We randomly divided 30 SD rats into three groups:control group,diabetes mellitus group (diabetic model rat induced by alloxan),APS group.After having been doctored for 5 weeks,the rats were killed and their blood was withdrawn by carotid artery catheter and used to measure the levels of glucose,serum insulin,SOD,MDA,ET and NO.Myocardiac pathologies of each group were used for morphometric studies. Result :Compared with control groups,the levels of glucose,MDA,NO,SOD and Ins were changed significantly.After administration of APS,the levels of Glu,MDA and ET decreased an the levels of NO,Ins and SOD increased in diabetic model groups.Under the microscope,the capillary density and the ratio of capillary to myofibre in DM rats were reduced remarkably compared with control group.The capillary basement membrance in DM rats was thicker.These alterations could be amended by APS. Conclusion :APS has the function of protecting the vessel endothelial cells of diabetes mellitus.